Sensory innervation of the guinea pig colon and rectum compared using retrograde tracing and immunohistochemistry

Autor:	DF Sharrad, Simon J. H. Brookes, B. N. Chen, Catharina Olsson
Rok vydání:	2016
Předmět:	0301 basic medicine Sensory Receptor Cells Colon Physiology Guinea Pigs Rectum Sensory system Calcitonin gene-related peptide Splanchnic nerves Guinea pig 03 medical and health sciences 0302 clinical medicine Animals Medicine Neuronal Tract-Tracers Endocrine and Autonomic Systems business.industry Gastroenterology Anatomy Immunohistochemistry Retrograde tracing digestive system diseases Neuroanatomical Tract-Tracing Techniques 030104 developmental biology medicine.anatomical_structure nervous system business 030217 neurology & neurosurgery Sacral ganglia
Zdroj:	Neurogastroenterology & Motility. 28:1306-1316
ISSN:	1350-1925
DOI:	10.1111/nmo.12825
Popis:	Background Neurons in lumbar and sacral dorsal root ganglia (DRG) comprise extrinsic sensory pathways to the distal colon and rectum, but their relative contributions are unclear. In this study, sensory innervation of the rectum and distal colon in the guinea pig was directly compared using retrograde labeling combined with immunohistochemistry. Methods The lipophilic tracer, DiI, was injected in either the rectum or distal colon of anesthetized guinea pigs, then DRG (T6 to S5) and nodose ganglia were harvested and labeled using antisera for calcitonin gene-related peptide (CGRP) and transient receptor potential vanilloid 1(TRPV1). Key Results More primary afferent cell bodies were labeled from the rectum than from the distal colon. Vagal sensory neurons, with cell bodies in the nodose ganglia comprised fewer than 0.5% of labeled sensory neurons. Spinal afferents to the distal colon were nearly all located in thoracolumbar DRG, in a skewed unimodal distribution (peak at L2); fewer than 1% were located in sacral ganglia. In contrast, spinal afferents retrogradely labeled from the rectum had a bimodal distribution, with one peak at L3 and another at S2. Fewer than half of all retrogradely labeled spinal afferent neurons were immunoreactive for CGRP or TRPV1 and these included the larger traced neurons, especially in thoracolumbar ganglia. Conclusions & Inferences In the guinea pig, both the distal colon and the rectum receive a sensory innervation from thoracolumbar ganglia. Sacral afferents innervate the rectum but not the distal colon. Calcitonin gene-related peptide immunoreactivity was detectable in fewer than half of afferent neurons in both pathways.
Databáze:	OpenAIRE
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