Detailed Analysis of Mucosal Restoration of the Small Intestine After the Cavitary Two‐Layer Cold Storage Method
| Autor: | Keitaro Kakinoki, Yasuki Tanioka, Yasuyuki Suzuki, Hiroshi Yokozaki, Yasuhiro Fujino, Yoshikazu Kuroda, Shiri Li, Tomohiro Tanaka, Tetsuya Sakai, Takuro Yoshikawa, Tadahiro Goto, Ippei Matsumoto |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors medicine.medical_treatment Preservation Biological Ischemia Cold storage Apoptosis Basal (phylogenetics) Dogs Microscopy Electron Transmission Proliferating Cell Nuclear Antigen Intestine Small In Situ Nick-End Labeling medicine Animals Immunology and Allergy Pharmacology (medical) Viaspan Intestinal Mucosa Cell Proliferation Transplantation Mucous Membrane business.industry Graft Survival Immunosuppression Organ Preservation Organ Transplantation Hypothermia medicine.disease Immunohistochemistry Small intestine Mitochondria Cold Temperature Microscopy Electron medicine.anatomical_structure Reperfusion Injury Reperfusion Female medicine.symptom business Immunosuppressive Agents |
| Zdroj: | American Journal of Transplantation. 5:2135-2142 |
| ISSN: | 1600-6135 |
| DOI: | 10.1111/j.1600-6143.2005.01001.x |
| Popis: | Small bowel transplantation (SBT) is associated with a high incidence of infectious complications because of ischemia/reperfusion (I/R) mucosal injury concomitant with potent immunosuppression. In this study, we evaluated whether the cavitary two-layer method (cTLM) could reduce I/R injury and allow early mucosal restoration, particularly after prolonged preservation and transplantation. Canine heterotopic segmental SBT was performed immediately without preservation (group 1), after 24-h preservation in UW solution (group 2) or by the cTLM (group 3). The graft samples were taken 1 h after reperfusion and on days 1, 4 and 7. We assessed graft mucosa with detailed microscopic and electromicroscopic analyses. In Group 3, histological injury and cell apoptosis after transplantation were significantly alleviated and rapidly recovered to a similar level of group 1. The mucosal restoration was morphologically completed within 4 days. In contrast, in group 2, more pronounced mucosal injury and delayed recovery were noted. Crypt cell proliferation activity was well maintained in groups 1 and 3 throughout the experimental period. Our ultrastructural analysis suggested that mitochondrial integrity achieved by the cTLM was a basal mechanism under the prompt mucosal restoration. The cTLM could reduce I/R injury, facilitate mucosal regeneration and restore the nearly normal structure early after SBT. |
| Databáze: | OpenAIRE |
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