Fine characterization of childhood and adult acute lymphoblastic leukemia (ALL) by a proB and preB surrogate light chain-specific mAb and a proposal for a new B cell ALL classification

Autor: B Lemmers, C Arnoulet, Danielle Sainty, C Schiff, Chantal Fossat, Jean Gabert, Hervé Chambost
Přispěvatelé: Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Rok vydání: 2001
Předmět:
Adult
Cancer Research
medicine.drug_class
Cell
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Monoclonal antibody
Immunoglobulin light chain
Immunophenotyping
03 medical and health sciences
0302 clinical medicine
Acute lymphocytic leukemia
medicine
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Child
ComputingMilieux_MISCELLANEOUS
B cell
030304 developmental biology
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
Antibodies
Monoclonal
Hematology
medicine.disease
Flow Cytometry
Phenotype
Molecular biology
Burkitt Lymphoma
medicine.anatomical_structure
Oncology
Cytoplasm
030220 oncology & carcinogenesis
Immunology
[SDV.IMM]Life Sciences [q-bio]/Immunology
Cytometry
Zdroj: Leukemia
Leukemia, Nature Publishing Group: Open Access Hybrid Model Option B, 2000, 14 (12), pp.2103-2111. ⟨10.1038/sj.leu.2401932⟩
ISSN: 0887-6924
1476-5551
Popis: The expression of the surrogate light chain (psiL) - made of the lambda-like (or lambda5) and the VpreB proteins - is a B cell-specific maturation marker. Using an anti-human VpreB mAb (4G7), we recently identified in human normal bone marrows, proB and preB cells that express the psiH-psiL proB (proBCR) and the mu-psiL preB (preBCR) receptors, respectively. In the present study, FACS and biochemical analysis confirm the broad proB and preB reactivity of the 4G7 mAb that contrasts with the narrow specificity of other available anti-psiL reagents for preB cells. This mAb was used to explore intracytoplasmic and cell surface expression of the VpreB protein on a series of 92 precursor B cell ALLs (from 40 child and 52 adult patients), in combination with 24 other mAbs. The major result concerns the identification within proB (or BI) and common (or BII) ALLs, of proBCR and proBCR+ ALLs that express the VpreB in the cytoplasm or at the cell surface, respectively. The percentage of ALLs within these two VpreB sub-groups differ considerably between the ALL origin. In the pediatric series, ALLs present in the majority a proBCR+ phenotype whereas we observed a proBCR+ phenotype for adult ALLs. Based on VpreB expression, and in combination with other published data, we propose a refined classification for precursor B cell ALLs.
Databáze: OpenAIRE
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