Proteomics Analysis of Plasma Membrane Fractions of the Root, Leaf, and Flower of Rice
| Autor: | Kotaro Miura, Yusuke Hagi, Yuki Kobayashi, Yukimoto Iwasaki, Takafumi Itoh, Aki Nishiyama, Sakura Matsuta, Setsuko Komatsu, Genki Chaya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine heterotrimeric G protein Immunoprecipitation organ specificity Flowers Endocytosis Proteomics plasma membrane Plant Roots 01 natural sciences Article Catalysis Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences proteomics Gene Expression Regulation Plant Heterotrimeric G protein Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Ion transporter Plant Proteins Chemistry rice Organic Chemistry fungi food and beverages Oryza General Medicine Computer Science Applications Plant Leaves 030104 developmental biology Membrane lcsh:Biology (General) lcsh:QD1-999 Etiolation Biophysics Signal transduction 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 6988, p 6988 (2020) International Journal of Molecular Sciences Volume 21 Issue 19 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The plasma membrane regulates biological processes such as ion transport, signal transduction, endocytosis, and cell differentiation/proliferation. To understand the functional characteristics and organ specificity of plasma membranes, plasma membrane protein fractions from rice root, etiolated leaf, green leaf, developing leaf sheath, and flower were analyzed by proteomics. Among the proteins identified, 511 were commonly accumulated in the five organs, whereas 270, 132, 359, 146, and 149 proteins were specifically accumulated in the root, etiolated leaf, green leaf, developing leaf sheath, and developing flower, respectively. The principle component analysis revealed that the functions of the plasma membrane in the root was different from those of green and etiolated leaves and that the plasma membrane protein composition of the leaf sheath was similar to that of the flower, but not that of the green leaf. Functional classification revealed that the root plasma membrane has more transport-related proteins than the leaf plasma membrane. Furthermore, the leaf sheath and flower plasma membranes were found to be richer in proteins involved in signaling and cell function than the green leaf plasma membrane. To validate the proteomics data, immunoblot analysis was carried out, focusing on four heterotrimeric G protein subunits, G&alpha G&beta G&gamma 1, and G&gamma 2. All subunits could be detected by both methods and, in particular, G&gamma 1 and G&gamma 2 required concentration by immunoprecipitation for mass spectrometry detection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|