Draft genome assembly of Passalora sequoiae a needle blight pathogen on Leyland cypress
| Autor: | Renee S. Arias, Valerie A. Orner, Warren E. Copes, Alan S. Windham, Ebrahiem M. Babiker, Jorge Ibarra Caballero, Jane Stewart |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Leyland cypress Mycosphaerellaceae Cupressocyparis leylandii Sequence assembly lcsh:Medicine Data Note Genome General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mississippi Ascomycota Illumina Botany Needle blight lcsh:Science (General) Genome size lcsh:QH301-705.5 Whole genome sequencing PacBio biology Contig lcsh:R High-Throughput Nucleotide Sequencing General Medicine Genome project Cupressus 030108 mycology & parasitology biology.organism_classification 030104 developmental biology lcsh:Biology (General) Genome annotation lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 13, Iss 1, Pp 1-5 (2020) BMC Research Notes |
| ISSN: | 1756-0500 |
| DOI: | 10.1186/s13104-020-05328-3 |
| Popis: | Objective Passalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses for ornamental plant nursery and Christmas tree producers. The objective was to generate a high-quality draft assembly of the genome of P. sequoiae as a resource for primer development to investigate genotype diversity. Data description We report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress ‘Leighton Green’ in 2017 in southern Mississippi, USA. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 × coverage and were used to confirm accuracy of the consensus sequences. |
| Databáze: | OpenAIRE |
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