3D spheroid cultures for evaluation of nanophotosensitizers accumulation
| Autor: | Victor B. Loschenov, L. N. Bolotine, A. A. Pankratov, Tatyana Karmakova, I.D. Romanishkin, Dina Farrakhova, Anna D. Plyutinskaya, Yulia Maklygina, Aleksey S. Skobeltsin, Anastasia V. Ryabova, Ilya Yakovets, Marie Millard |
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| Přispěvatelé: | A. M. Prokhorov General Physics Institute (GPI), Russian Academy of Sciences [Moscow] (RAS), Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), National Institute for Medical Research - Muhimbili Research Centre (NIMR), The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
History Chemistry [SDV]Life Sciences [q-bio] Tumor spheroid Cell Spheroid Nanoparticle 3d model 02 engineering and technology 021001 nanoscience & nanotechnology Computer Science Applications Education 03 medical and health sciences chemistry.chemical_compound medicine.anatomical_structure Organelle Monolayer Biophysics medicine 0210 nano-technology Indocyanine green 030304 developmental biology |
| Zdroj: | VIII International Youth Scientific School-Conference "Modern Problems of Physics and Technology", MPPT2019 VIII International Youth Scientific School-Conference "Modern Problems of Physics and Technology", MPPT2019, Apr 2019, Moscou, Russia. pp.012032, ⟨10.1088/1742-6596/1439/1/012032⟩ |
| Popis: | Published in Journal of Physics: Conference Series, 1439:012032, IOPScience, 2020; International audience; Current paper presents the results of the usage of indocyanine green and pheophorbide nanoform on 2D and 3D models of FaDu cells culture. The 2D model or monolayer was used for investigation of nanoparticles distribution within individual cells and their organelles. The 3D model or multicellular tumor spheroids were used for estimation of cells' metabolic processes by the investigation of the nanophotosensitizers fluorescence distribution within spheroid's layers. It was shown that pheophorbide nanoparticles are accumulated in the external cell layers of spheroids, indocyanine green nanoparticles distribution demonstrates completely opposite status – in the central part of the spheroid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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