Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe
| Autor: | Ramapurath S. Jayasree, Resmi V. Nair, Ayyappanpillai Ajayaghosh, Divya Susan Philips, Hema Santhakumar, Anoopkumar Thekkuveettil, Sachin J. Shenoy |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
lcsh:Medicine chemistry.chemical_element Endogeny 02 engineering and technology Zinc Hippocampal formation 03 medical and health sciences 0302 clinical medicine Postsynaptic potential In vivo Microscopy lcsh:Science Multidisciplinary Chemistry lcsh:R 021001 nanoscience & nanotechnology Fluorescence In vitro enzymes and coenzymes (carbohydrates) biological sciences health occupations Biophysics bacteria lcsh:Q 0210 nano-technology 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-27029-5 |
| Popis: | Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn2+ distribution and its dynamics are essential in view of the fact that Zn2+ dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn2+ in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn2+. The probe is used to detect and quantify the exogenous and endogenous Zn2+ in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons, in vitro wherein the translocation of Zn2+ from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn2+ under epileptic condition. Finally, the Zn2+ distribution was imaged in vivo in epileptic rats and the total Zn2+ in rat brain was quantified. The results favour the use of BP as an excellent Zn2+ imaging probe in biological system to understand the zinc associated diseases and their management. |
| Databáze: | OpenAIRE |
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