Update on mammalian sperm capacitation: How much does the horse differ from other species?
| Autor: | Leemans, Bart, Stout, Tom Arjun Edgar, De Schauwer, Catharina, Heras, Sonia, Nelis, Hilde, Hoogewijs, Maarten, Van Soom, Ann, Gadella, Bart M, dES/dFAH FR, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten, dB&C FR-RMSC FR, Sub Reproductie mannelijk, Sub Biologie van de mannelijke gameet |
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| Přispěvatelé: | dES/dFAH FR, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten, dB&C FR-RMSC FR, Sub Reproductie mannelijk, Sub Biologie van de mannelijke gameet |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Embryology endocrine system medicine.medical_treatment Acrosome reaction 03 medical and health sciences 0302 clinical medicine Endocrinology Species Specificity Capacitation medicine Animals Humans Horses education Zona pellucida Lipid raft reproductive and urinary physiology Sperm plasma membrane Mammals education.field_of_study 030219 obstetrics & reproductive medicine In vitro fertilisation Chemistry urogenital system Acrosome Reaction Obstetrics and Gynecology Cell Biology Soluble adenylyl cyclase Spermatozoa Sperm Cell biology 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Fertilization Female Sperm Capacitation |
| Zdroj: | Reproduction, 157(5), R181. BioScientifica Ltd. |
| ISSN: | 1470-1626 |
| DOI: | 10.1530/rep-18-0541 |
| Popis: | In contrast to various other mammalian species, conventionalin vitrofertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fails to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) underin vitroconditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Nevertheless, knowledge of capacitation pathways and their molecular triggers might improve our understanding of capacitation-related events observed in stallion sperm. When sperm cells are exposed to appropriate capacitation triggers, several molecular and biochemical changes should be induced in the sperm plasma membrane and cytoplasm. At the level of the sperm plasma membrane, (1) an increase in membrane fluidity, (2) cholesterol depletion and (3) lipid raft aggregation should occur consecutively; the cytoplasmic changes consist of protein tyrosine phosphorylation and elevated pH, cAMP and Ca2+concentrations. These capacitation-related events enable the switch from progressive to hyperactivated motility of the sperm cells, and the induction of the acrosome reaction. These final capacitation triggers are indispensable for sperm cells to migrate through the viscous oviductal environment, penetrate the cumulus cells and zona pellucida and, finally, fuse with the oolemma. This review will focus on molecular aspects of sperm capacitation and known triggers in various mammalian species. Similarities and differences with the horse will be highlighted to improve our understanding of equine sperm capacitation/fertilizing events. |
| Databáze: | OpenAIRE |
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