Activity Patterns Govern Synapse-Specific AMPA Receptor Trafficking between Deliverable and Synaptic Pools
| Autor: | Genrieta Bochorishvili, J. Julius Zhu, Lei Zhang, Diane L. Rosin, Anders Kielland, James Corson, Paul Heggelund |
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| Rok vydání: | 2009 |
| Předmět: |
Patch-Clamp Techniques
Neuroscience(all) Green Fluorescent Proteins Models Neurological Tetrodotoxin AMPA receptor In Vitro Techniques Neurotransmission Biology Hippocampus Synaptic Transmission MOLNEURO Statistics Nonparametric Article Membrane Potentials Synapse Mice Transduction Genetic Ca2+/calmodulin-dependent protein kinase Animals Receptors AMPA Anesthetics Local Enzyme Inhibitors Microscopy Immunoelectron Mice Knockout Neurons Synaptic pharmacology musculoskeletal neural and ocular physiology General Neuroscience Excitatory Postsynaptic Potentials Geniculate Bodies Rats Transport protein Protein Subunits Protein Transport Animals Newborn nervous system SIGNALING Synapses ras Proteins Excitatory postsynaptic potential CELLBIO Calcium-Calmodulin-Dependent Protein Kinase Type 2 Neuroscience Postsynaptic density Signal Transduction |
| Zdroj: | Neuron. 62:84-101 |
| ISSN: | 0896-6273 |
| Popis: | SummaryIn single neurons, glutamatergic synapses receiving distinct afferent inputs may contain AMPA receptors (-Rs) with unique subunit compositions. However, the cellular mechanisms by which differential receptor transport achieves this synaptic diversity remain poorly understood. In lateral geniculate neurons, we show that retinogeniculate and corticogeniculate synapses have distinct AMPA-R subunit compositions. Under basal conditions at both synapses, GluR1-containing AMPA-Rs are transported from an anatomically defined reserve pool to a deliverable pool near the postsynaptic density (PSD), but further incorporate into the PSD or functional synaptic pool only at retinogeniculate synapses. Vision-dependent activity, stimulation mimicking retinal input, or activation of CaMKII or Ras signaling regulated forward GluR1 trafficking from the deliverable pool to the synaptic pool at both synapses, whereas Rap2 signals reverse GluR1 transport at retinogeniculate synapses. These findings suggest that synapse-specific AMPA-R delivery involves constitutive and activity-regulated transport steps between morphological pools, a mechanism that may extend to the site-specific delivery of other membrane protein complexes. |
| Databáze: | OpenAIRE |
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