Cellular Localization of Isoprenoid Biosynthetic Enzymes inMarchantia polymorpha. Uncovering a New Role of Oil Bodies
| Autor: | Florence Bouvier, Ralph A. Backhaus, Bilal Camara, Claude Suire, Dominique Bégu, Marc Bonneu |
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| Přispěvatelé: | Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
Phytoene desaturase Physiology [SDV]Life Sciences [q-bio] Plant Science 01 natural sciences 03 medical and health sciences Marchantia polymorpha Hemiterpenes Farnesyl diphosphate synthase Pentanes Plant Cells Butadienes Oils Volatile Genetics Plant Oils Fluorescent Antibody Technique Indirect Cellular localization 030304 developmental biology Organelles 0303 health sciences Farnesyltranstransferase Phytoene synthase biology ATP synthase Plants biology.organism_classification Geranyltranstransferase Cell Compartmentation Microscopy Electron Biochemistry biology.protein Research Article 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2000, 124 (3), pp.971-978. ⟨10.1104/pp.124.3.971⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.124.3.971 |
| Popis: | Like seed plants, liverworts synthesize and accumulate a myriad of isoprenoid compounds. Using antibodies raised against several isoprenoid biosynthetic enzymes, we investigated their intracellular compartmentation by in situ immunolocalization from Marchantia polymorpha. The enzymes examined were deoxy-xylulose phosphate synthase, geranyl diphosphate synthase, farnesyl diphosphate synthase, geranylgeranyl diphosphate synthase, monoterpene synthase, geranylgeranyl diphosphate reductase, phytoene synthase, and phytoene desaturase. Our results show that liverwort oil bodies, which are organelles bound by a single unit membrane, possess isoprenoid biosynthetic enzymes similar to those found in plastids and the cytosol. We postulate that oil bodies play a dynamic role in cell metabolism in addition to their role as sites of essential oil accumulation and sequestration. The occurrence of such enzymes in different cellular compartments might be due to multiple targeting of gene products to various organelles. |
| Databáze: | OpenAIRE |
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