Abnormalities in the p34cdc2-Related PITSLRE Protein Kinase Gene Complex (CDC2L) on Chromosome Band 1p36 in Melanoma
| Autor: | Raymond Taetle, Marianne Broome-Powell, Jin Ming Yang, FH Thompson, Maria E. Ariza, Mark A. Nelson, Julie Wymer, Jeffrey M. Trent, Vincent J. Kidd, Kathy Massey-Brown, Jill M. Lahti, John Easton |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Tumor suppressor gene Locus (genetics) Protein Serine-Threonine Kinases Biology medicine.disease_cause Polymerase Chain Reaction Gene expression Tumor Cells Cultured Genetics medicine Humans Protein kinase A Melanoma Molecular Biology Gene In Situ Hybridization Polymorphism Single-Stranded Conformational Base Sequence Promoter DNA Neoplasm Cell cycle Molecular biology Cyclin-Dependent Kinases Chromosomes Human Pair 1 Mutation Carcinogenesis Protein Kinases |
| Zdroj: | Cancer Genetics and Cytogenetics. 108:91-99 |
| ISSN: | 0165-4608 |
| Popis: | The two genes encoding the PITSLRE protein kinase isoforms, CDC2L1 and CDC2L2, are localized to human chromosome band 1p36. The PITSLRE protein kinases are a part of the p34cdc2 supergene family. Several protein products of the CDC2L locus may be effector(s) in apoptotic signaling. The larger PITSLRE p110 isoforms appear to regulate some aspect of RNA splicing/transcription during the cell cycle. One or more of these genes may function as tumor suppressor genes in melanoma. Using fluorescence in situ hybridization, one allele of the CDC2L gene complex on chromosome 1 was either deleted or translocated in 8 of 14 different melanoma cell lines. We also observed mutations in the 5' promoter region of the CDC2L1 gene in four different cell lines relative to normal melanocytes using PCR-SSCP analysis and direct DNA sequencing. Western blot analysis revealed decreased level of PITSLRE protein expression in several cell lines, as well as in four surgical malignant melanoma specimens relative to normal melanocytes. Thus, the decreased PITSLRE protein expression appears to result from deletion of the CDC2L alleles and possibly by mutations within the 5' promoter region. We propose that aberrations in the CDC2L genes may contribute to the pathogenesis or progression of melanoma. |
| Databáze: | OpenAIRE |
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