The effects of platelet-rich plasma on the osteogenic induction of bone marrow mesenchymal stem cells
| Autor: | Huilin Yang, Chunshen Wu, Cheng Cao, Jun Zou, Chenxi Yuan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Bone Marrow Cells
Biochemistry stomatognathic system Rheumatology Tissue engineering Osteogenesis Animals Orthopedics and Sports Medicine MTT assay Osteopontin Molecular Biology Cells Cultured biology Platelet-Rich Plasma Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Molecular biology Multipotent Stem Cell Platelet-rich plasma Immunology Osteocalcin biology.protein Alkaline phosphatase Rabbits |
| Zdroj: | Connective Tissue Research. 55:304-309 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.3109/03008207.2014.930140 |
| Popis: | Bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells. Finding methods to improve the osteogenic potential of these cells is a key factor in bone tissue engineering. Platelet-rich plasma (PRP) contains powerful growth factors that produce changes in a variety of cell types. The purpose of this study was to explore the effects of PRP on the osteogenic differentiation of BMSCs in vitro. Rabbit BMSCs were harvested and cultured in vitro in control media or in media enhanced with PRP. BMSCs began to attach 12-24 hours after seeding. A MTT assay demonstrated that PRP-induced BMSCs grew rapidly compared with the control group. The PRP group also showed strongly positive staining of alkaline phosphatase and mineralized nodules whereas the control group showed negative staining. However, the alkaline phosphatase activity and the mRNA level of the osteogenic markers (osteocalcin and osteopontin) remained higher in the PRP group. These results confirmed that PRP could enhance the proliferation of BMSCs and effectively promote the osteogenic differentiation of BMSCs in vitro. |
| Databáze: | OpenAIRE |
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