Neural cells in culture: models for the purification and the study of the effects of growth factors
| Autor: | Marston Manthorpe, Brigitte Pettmann |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Continuous measurement
Health Toxicology and Mutagenesis medicine.medical_treatment Cell Biology Toxicology chemistry.chemical_compound medicine Animals Bioassay Growth Substances Cells Cultured Neurons Isoelectric focusing Growth factor Biological activity Cell Biology Cell biology Molecular Weight medicine.anatomical_structure chemistry Cell culture Immunology Agarose Biological Assay Mitogens Neuroglia |
| Zdroj: | Cell Biology and Toxicology. 8:107-115 |
| ISSN: | 1573-6822 0742-2091 |
| Popis: | We have summarized several methodologies currently available for the detection and analysisof protein factors affecting neural cells. As more knowledge is gained concerning growthfactors and their influences on cells and as the demand for bioassays increases, methodologicalimprovements are likely to be aimed at 1) decreasing yet the assay time; 2) increasingsensitivity of the measurements of cell responses; 3) increasing the number of simultaneouscellular responses measurable. A new very promising technique involves the use of severalfluorochromes having different cellular localizations and the continuous measurement of theirevolution in living cells by fluorospectrophotometry.The cell-blot method can be expected to extend to fractions submitted to other forms ofchromatography (native electrophoresis, isoelectric focusing, agarose). Other modificationsmay allow the detection of antagonistic factors, by including a stimulatory factor in the blotculture medium and measuring the suppression of a cellular response by the blotted opposingactivity. |
| Databáze: | OpenAIRE |
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