Muscarinic cholinergic receptors in lung of developing rats
| Autor: | Paola Bernard, Claudio Bencini, Maria Carrara, Nolita Pulerá, Gian Maria Pacifici |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Aging Biology Fetus Pregnancy Internal medicine Muscarinic acetylcholine receptor Muscarinic acetylcholine receptor M4 medicine Radioligand Animals Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Lung Acetylcholine receptor Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Rats Inbred Strains Muscarinic acetylcholine receptor M1 Receptors Muscarinic Rats Dissociation constant Quinuclidinyl Benzilate Endocrinology Animals Newborn Female |
| Zdroj: | Developmental pharmacology and therapeutics. 11(3) |
| ISSN: | 0379-8305 |
| Popis: | The muscarin-specific radioligand 1-quinuclidinyl-[phenyl-4-3H]-benzilate was used to determine the development of muscarinic receptors in particulate membranes of rat lung. Postnatal development is associated with a decrease in the number of binding sites (Bmax). Bmax was 65.61 +/- 11.33 fmol/mg protein in 21-day-old rat fetuses and it decreased to 36.64 +/- 10.18 at postnatal day 1, 32.37 +/- 4.99 at day 7 and 16.15 +/- 4.13 fmol/mg protein at 2 months. Bmax at 2 months was significantly different from all the others (Dunnett's test). The dissociation constant Kd was 0.25 nM in 2-month-old animals and it did not undergo significant changes during development. |
| Databáze: | OpenAIRE |
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