Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture

Autor: Karoly Szuhai, Philippe Gallay, Udayan Chatterji, Eric J. Snijder, Danielle de Jong, Adriaan H. de Wilde, Jessika C. Zevenhoven-Dobbe, Clara C. Posthuma, Corrine Beugeling
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Virology
Virology, 517, 148-156
ISSN: 1096-0341
0042-6822
Popis: Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.
Highlights • Nidoviruses display differences in sensitivity towards cyclophilin A depletion. • Replication of MERS-coronavirus is reduced modestly in cyclophilin A-knockout cells. • Equine arteritis virus replication is strongly inhibited by cyclophilin A depletion. • Chromosomal anomalies complicate CRISPR/Cas9-mediated gene editing in Huh7 cells.
Databáze: OpenAIRE
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