High-Resolution Hyperpolarized In Vivo Metabolic 13C Spectroscopy at Low Magnetic Field (48.7 mT) Following Murine Tail-Vein Injection
| Autor: | Matthew A. Feldman, Roman V. Shchepin, Aaron M. Coffey, Eduard Y. Chekmenev, Danila A. Barskiy, Wellington Pham, Milton L. Truong |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Tail
Nuclear and High Energy Physics Magnetic Resonance Spectroscopy Biophysics Contrast Media Mice Nude 010402 general chemistry Spin isomers of hydrogen 01 natural sciences Biochemistry Article 030218 nuclear medicine & medical imaging Veins 03 medical and health sciences Laser linewidth Mice 0302 clinical medicine Nuclear magnetic resonance Electromagnetic Fields In vivo Cell Line Tumor Animals Hyperpolarization (physics) Spectroscopy Carbon Isotopes Chemistry Resonance Neoplasms Experimental Carbon-13 NMR Condensed Matter Physics Magnetic Resonance Imaging 0104 chemical sciences Full width at half maximum Injections Intravenous Female |
| Popis: | High-resolution 13C NMR spectroscopy of hyperpolarized succinate-1-13C is reported in vitro and in vivo using a clinical-scale, biplanar (80 cm-gap) 48.7 mT permanent magnet with a high homogeneity magnetic field. Non-localized 13C NMR spectra were recorded at 0.52 MHz resonance frequency over the torso of a tumor-bearing mouse every 2 seconds. Hyperpolarized 13C NMR signals with linewidths of ~3 Hz (corresponding to ~6 ppm) were recorded in vitro (2 mL in a syringe) and in vivo (over a mouse torso). Comparison of the full width at half maximum (FWHM) for 13C NMR spectra acquired at 48.7 mT and at 4.7 T in a small-animal MRI scanner demonstrates a factor of ~12 improvement for the 13C resonance linewidth attainable at 48.7 mT compared to that at 4.7 T in vitro. 13C hyperpolarized succinate-1-13C resonance linewidths in vivo are at least one order of magnitude narrower at 48.7 mT compared to those observed in high-field (≥ 3 T) studies employing HP contrast agents. The demonstrated high-resolution 13C in vivo spectroscopy could be useful for high-sensitivity spectroscopic studies involving monitoring HP agent uptake or detecting metabolism using HP contrast agents with sufficiently large 13C chemical shift differences. |
| Databáze: | OpenAIRE |
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