Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model

Autor: Rafael Jurado, Jazmin M. Pérez-Rojas, Raquel González-Macías, Patricia García-López, José Pedraza-Chaverri, Jaime González-Cortes
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Aging
Time Factors
Uterine Cervical Neoplasms
Apoptosis
Pharmacology
Biochemistry
HeLa
0302 clinical medicine
Medicine
Drug Interactions
Cytotoxicity
chemistry.chemical_classification
Mice
Inbred BALB C
biology
lcsh:Cytology
Cell Cycle
Drug Synergism
General Medicine
Cell cycle
female genital diseases and pregnancy complications
Tumor Burden
030220 oncology & carcinogenesis
Female
medicine.drug
Research Article
inorganic chemicals
Article Subject
Cell Survival
Xanthones
Mice
Nude
Nephrotoxicity
03 medical and health sciences
In vivo
Animals
Humans
lcsh:QH573-671
neoplasms
Cell Proliferation
Cisplatin
Reactive oxygen species
business.industry
Cell Biology
biology.organism_classification
030104 developmental biology
chemistry
business
Reactive Oxygen Species
Biomarkers
HeLa Cells
Zdroj: Oxidative Medicine and Cellular Longevity, Vol 2016 (2016)
Oxidative Medicine and Cellular Longevity
ISSN: 1942-0994
1942-0900
Popis: Cervical cancer is the second leading cause of death among Mexican women. The treatment with cis-diamminedichloroplatinum (II) (CDDP) has some serious side effects.Alpha-mangostin (α-M), has a protective effect against CDDP-induced nephrotoxicity, as well as antioxidant, antitumor, and anti-inflammatory properties. Hence, we explored the in vitro and in vivo effect ofα-M on human cervical cancer cell proliferation when combined with CDDP. In vitro, The cytotoxic effect ofα-M and/or CDDP was measured by the 3-(3,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium assay. Meanwhile, apoptosis, reactive oxygen species (ROS) production, and the cell cycle were determined with flow cytometry. Forα-M+CDDP treatment, both a coincubation and preincubation scheme were employed. In vivo, xenotransplantation was performed in female athymic BALB/c (nu/nu) mice, and then tumor volume and body weight were measured weekly, whereasα-M interfered with the antiproliferative activity of CDDP in the coincubation scheme, with preincubation withα-M+CDDP showing significantly greater cytotoxicity than CDDP orα-M alone, significantly inhibiting average tumor volume and preventing nephrotoxicity. This effect was accompanied by increased apoptosis and ROS production by HeLa cervical cancer cells, as well as an arrest in the cell cycle. These results suggest thatα-M may be useful as a neoadjuvant agent in cervical cancer therapy.
Databáze: OpenAIRE
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