Assessment of a pro-healing stent in an animal model of early neoatherosclerosis
| Autor: | Tobias Koppara, Garry Kerch, Tobias Lenz, Liang Guo, Petra Hoppmann, Roisin Colleran, Kristina Euller, Qi Cheng, Michael Joner, Michael Weinmüller, Christoph Lutter, Eduardo Acampado, Florian Rechenmacher, Anna Lena Lahmann, Horst Kessler, Maria Isabel Castellanos, Anna Bulin, Stefanie Neubauer, Beatrice Stefanie Ludwig, Philipp Nicol, Kristin Steigerwald |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment lcsh:Medicine Vascular permeability 030204 cardiovascular system & hematology Article 03 medical and health sciences 0302 clinical medicine Animal model medicine Animals cardiovascular diseases lcsh:Science Foam cell Wound Healing Multidisciplinary Everolimus business.industry lcsh:R Stent 030208 emergency & critical care medicine Atherosclerosis ddc Endothelial stem cell Disease Models Animal Cardiovascular diseases Permeability (electromagnetism) Cell culture Stents lcsh:Q Rabbits business Tunica Intima Interventional cardiology medicine.drug Foam Cells |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
| Popis: | Background: Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits. Methods and Results: Neointimal foam cell formation was induced in rabbits (n = 7). Enhanced endothelial integrity in RGD-coated stents resulted in decreased vascular permeability relative to DES, which was further confirmed by SEM and TEM. Cell culture experiments examined the effect of everolimus on endothelial integrity. Increasing concentrations of everolimus resulted in a dose-dependent decrease of endothelial cell junctions and foam cell transformation of monocytes, confirming the relevance of endothelial integrity in preventing permeability of LDL. Conclusion: Incomplete endothelial integrity was confirmed as a key factor of neointimal foam cell formation following stent implantation. Pro-healing stent coatings may facilitate reendothelialization and reduce the risk of neoatherosclerosis. |
| Databáze: | OpenAIRE |
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