Contribution of High Viral Loads, Detection of Viral Antigen and Seroconversion to Severe Acute Respiratory Syndrome Coronavirus 2 Infectivity
| Autor: | Markus Bauswein, Thomas Müller, Matthias Lubnow, Felix Buder, Frank Hanses, Jürgen J. Wenzel, Christof Kundel, E Reuschel, Franz Audebert, Benedikt M J Lampl, Martina Müller, Karin Distler, Bernhard M. Graf, Clara L Magnus, Bernd Salzberger, Henriette Lang, Hendrik Poeck, David Peterhoff, Dirk Lunz, Barbara Schmidt, S Schmid |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male viruses Context (language use) Antibodies Viral Severity of Illness Index law.invention law Major Article Immunology and Allergy Medicine Humans viral antigen Seroconversion Respiratory system Antigens Viral Infectivity biology business.industry infectivity SARS-CoV-2 COVID-19 Odds ratio Viral Load Virology Intensive care unit Infectious Diseases AcademicSubjects/MED00290 biology.protein RNA Viral Female Public Health Antibody business Viral load |
| Zdroj: | The Journal of Infectious Diseases |
| ISSN: | 1537-6613 |
| Popis: | Background From a public health perspective, effective containment strategies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) should be balanced with individual liberties. Methods We collected 79 respiratory samples from 59 patients monitored in an outpatient center or in the intensive care unit of the University Hospital Regensburg. We analyzed viral load by quantitative real-time polymerase chain reaction, viral antigen by point-of-care assay, time since onset of symptoms, and the presence of SARS-CoV-2 immunoglobulin G (IgG) antibodies in the context of virus isolation from respiratory specimens. Results The odds ratio for virus isolation increased 1.9-fold for each log10 level of SARS-CoV-2 RNA and 7.4-fold with detection of viral antigen, while it decreased 6.3-fold beyond 10 days of symptoms and 20.0-fold with the presence of SARS-CoV-2 antibodies. The latter was confirmed for B.1.1.7 strains. The positive predictive value for virus isolation was 60.0% for viral loads >107 RNA copies/mL and 50.0% for the presence of viral antigen. Symptom onset before 10 days and seroconversion predicted lack of infectivity with negative predictive values of 93.8% and 96.0%. Conclusions Our data support quarantining patients with high viral load and detection of viral antigen and lifting restrictive measures with increasing time to symptom onset and seroconversion. Delay of antibody formation may prolong infectivity. |
| Databáze: | OpenAIRE |
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