Vaginal Microbiota and Mucosal Immune Markers in Women With Vulvovaginal Discomfort
| Autor: | Ollivier Hyrien, Alissa J. Mitchell, Sujatha Srinivasan, Agnes Bergerat, Noah G. Hoffman, Caroline M. Mitchell, Alisa Pascale, D.J. Valint, David N. Fredricks, LaTina J Watson |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Adult medicine.medical_specialty Dermatology medicine.disease_cause Immunoglobulin E Gastroenterology Article 03 medical and health sciences 0302 clinical medicine Internal medicine RNA Ribosomal 16S medicine Gardnerella vaginalis Humans 030212 general & internal medicine Microbiome Lactobacillus crispatus Vaginitis Inflammation Univariate analysis 030505 public health biology business.industry Sequence Analysis RNA Microbiota Public Health Environmental and Occupational Health Vaginosis Bacterial Middle Aged biology.organism_classification medicine.disease Infectious Diseases Case-Control Studies Vagina biology.protein Vulvodynia Cytokines Female Antibody 0305 other medical science business Biomarkers |
| Zdroj: | Sex Transm Dis |
| ISSN: | 1537-4521 |
| Popis: | Background Up to 30% of women with vaginal symptoms are not assigned a diagnosis after standard diagnostic assessment. Methods We compared premenopausal women with idiopathic vaginitis (IV) or vulvodynia (VVD) to healthy controls. Microbiota were characterized using rRNA sequencing. Cytokines/chemokines (IL-10, IL-1α, IL-1β, IL-6, IL-8, IL-2, IL-18, IL-4, IL-9, and IL-13) were measured in vaginal lavage fluid using the Meso Scale Discovery platform or ELISA (IL-1ra). Immunoglobulins were measured in vaginal lavage fluid using a bead-based immunoassay (Millipore). Cases and controls were compared using Kruskal-Wallis, analysis of variance, and linear regression or (for microbiome composition) the Bray-Curtis dissimilarity statistic. Results We compared 20 women with IV, 30 with VVD, and 52 controls. Most (80%) had greater than 90% 16S rRNA gene sequences from Lactobacillus crispatus, L. jensenii, L. gasseri, or L. iners. In analyses adjusted for age and hormonal contraception (HC), Gardnerella vaginalis was less prevalent and abundant in women with VVD (2/30, 7%) versus controls (16/52, 31%) or IV (5/20, 25%) (P = 0.030). Bray-Curtis dissimilarity was not significantly different between IV and controls or VVD. Fungal sequences were only detected in 5 participants: 2 control, 1 IV, 2 VVD. In univariate analysis, cytokines were not associated with diagnosis. Median vaginal concentration of IgE (but not other immunoglobulins) was lower in women with VVD (P = 0.006). Conclusions Minimal differences in vaginal microbiota and inflammatory markers between women with IV, VVD or controls suggest no striking association between vaginal bacteria, fungi or inflammation and diagnosis in these women. |
| Databáze: | OpenAIRE |
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