Evaluating the efficacy of a centrifugation-flotation method for extractingAscarisova from soil
| Autor: | Penelope J. Teoh, Jeroen H. J. Ensink, Imogen Cranston, Sarah M. Baker, Mita E. Sengupta |
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| Rok vydání: | 2016 |
| Předmět: |
Rural Population
Soil texture 030231 tropical medicine 0208 environmental biotechnology Helminthiasis Centrifugation 02 engineering and technology Biology complex mixtures Soil 03 medical and health sciences 0302 clinical medicine Animal science Helminths Animals Humans Organic matter Sanitation Toilet Facilities Ovum chemistry.chemical_classification Ascaris Ecology Soil organic matter Public Health Environmental and Occupational Health Soil classification General Medicine Contamination biology.organism_classification Soil type 020801 environmental engineering Infectious Diseases chemistry Parasitology |
| Zdroj: | Transactions of The Royal Society of Tropical Medicine and Hygiene. 110:400-407 |
| ISSN: | 1878-3503 0035-9203 |
| DOI: | 10.1093/trstmh/trw044 |
| Popis: | BACKGROUND Soil transmitted helminths (STH) continue to be associated with high burdens of disease, with an estimated 1.45 billion people infected with STH globally. The promotion and construction of latrines is considered the first barrier to prevent transmission of STH. The absence of a reliable method to extract STH ova from soil makes it challenging to examine whether the use of latrines may or may not have an effect on environmental contamination with ova. The present study evaluated the recovery rate of a method developed to extract STH ova from soil. METHODS The adapted centrifugation and flotation technique was applied to 15 soil types, which were seeded with Ascaris suum ova. Soil type, soil moisture content, soil texture and organic matter content were assessed for each soil sample. RESULTS The average ova recovery rate was 28.2%, with the recovery rate of the method decreasing with increasing soil moisture content, particle size and organic matter content. The association between recovery rate and organic matter content was statistically significant. CONCLUSIONS The present study identified a low recovery rate for an adapted centrifugation-flotation method, although this was similar to the recovery rate demonstrated by other methods developed for soil. Soil organic matter content was significantly associated with ova recovery rates. |
| Databáze: | OpenAIRE |
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