Prevalence of Major Methicillin-Resistant Staphylococcus aureus Clones in Korea Between 2001 and 2008

Autor: Jeong Ok Cha, Yeong Seon Lee, Gi Su Kang, Yung Hee Jung, Chan Park, Hwa Su Kim, Kwang Jun Lee
Rok vydání: 2016
Předmět:
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
medicine.medical_specialty
030106 microbiology
Clinical Biochemistry
Multilocus sequence typing
Biology
medicine.disease_cause
Staphylococcal infections
Genetic diversity
Microbiology
law.invention
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
law
Molecular genetics
Republic of Korea
Prevalence
medicine
Humans
030212 general & internal medicine
Typing
Polymerase chain reaction
Genetics
SCCmec
Biochemistry (medical)
Korean MRSA clones
General Medicine
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
medicine.disease
Methicillin-resistant Staphylococcus aureus
Hospitals
Electrophoresis
Gel
Pulsed-Field
Clinical Microbiology
Staphylococcus aureus
Original Article
Multiplex Polymerase Chain Reaction
DiversiLab
Zdroj: Annals of Laboratory Medicine
ISSN: 2234-3814
2234-3806
Popis: Background Methicillin-resistant Staphylococcus aureus (MRSA) are important pathogens causing nosocomial infections in Korean hospitals. This study aimed to investigate the epidemiological and genetic diversity of clinical S. aureus isolates in healthcare settings from 2001 to 2008. Methods Samples and data were obtained from 986 individuals as part of the National Antimicrobial Surveillance Project, involving 10 regions nationwide. Molecular typing studies, including multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were performed, and a representative clone of Korean MRSA was classified by combinational grouping using a DiversiLab (DL; bioMérieux, France) repetitive element polymerase chain reaction (rep-PCR) system. Results Nine Korean MRSA clones (KMRSA-1 to -9) were identified by analysis of genetic backgrounds and molecular characteristics. KMRSA-1 to -3, expressing clonal complex (CC) 5 (carrying SCCmec II), CC8 (carrying SCCmec III), and CC72 (carrying SCCmec IV) were spread nationwide. In contrast, KMRSA-6 was highly prevalent in Gyeongsangnam-do, and KMRSA-4 was highly prevalent in Jeollanam-do and Jeollabuk-do. Conclusions Epidemic KMRSA clones were genetically similar to major clones identified from the USA, with the exception of KMRSA-2, which had the SCCmec III type. Our results provide important insights into the distribution and molecular genetics of MRSA strains in Korea and may aid in the monitoring of MRSA spread throughout the country.
Databáze: OpenAIRE
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