Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

Autor: David Diouf, Maria Rosaria Vitale, Johanna Eva Maria Zöller, Ana-Magdalena Pineau, Eva Klopocki, Catharina Hamann, Georg Christoph Ziegler, Tim Vanmierlo, Daniel Van den Hove, Klaus-Peter Lesch
Přispěvatelé: Vitale, Maria Rosaria/0000-0002-1108-0523, Diouf, David/0000-0002-1728-5319, DIOUF, David, Vitale, Maria Rosaria, Zoeller, Johanna Eva Maria, Pineau, Ana -Magdalena, Klopocki, Eva, Hamann, Catharina, Ziegler, Georg Christoph, VANMIERLO, Tim, Van den Hove, Daniel, Lesch, Klaus-Peter, RS: MHeNs - R3 - Neuroscience, Basic Neuroscience 2, Basic Neuroscience 1, Psychiatrie & Neuropsychologie
Jazyk: angličtina
Předmět:
Zdroj: Stem Cell Research
Stem Cell Research, 67:103038. Elsevier
ISSN: 1873-5061
1010-8645
DOI: 10.1016/j.scr.2023.103038
Popis: Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity. This work was supported by ERA-NET NEURON under Grant No. 01EW1902 (DECODE!), the Horizon 2020 Research and Innovation Programme under Grant No. 728018 (Eat2beNICE), Grant No. 953327 (Serotonin and Beyond), the Horizon Europe Research and Innovation Programme under Grant No. EU 101086453 (Aqua-Synapse), the DFG (Grant No. 413657723, Clinician Scientist-Program UNION CVD), and the University of Würzburg in the funding program Open Access Publishing. We thank Julia Merk, Gabriela Ortega, and Nicole Schraud for excellent technical assistance.
Databáze: OpenAIRE
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