Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons

Autor: Marta Orlando, Felicitas Bruentgens, Jörg Breustedt, Benjamin R. Rost, Stephan J. Sigrist, Dietmar Schmitz, Marta Maglione, Anton Dvorzhak
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Physiology
Entropy
Distance Measurement
Biochemistry
Nervous System
Hippocampus
chemistry.chemical_compound
Nerve Fibers
Animal Cells
pharmacology [Colforsin]
Medicine and Health Sciences
Biology (General)
drug effects [Synaptic Vesicles]
Hippocampal mossy fiber
Neurons
Neurotransmitter Agents
Measurement
Forskolin
Voltage-dependent calcium channel
metabolism [Mossy Fibers
Hippocampal]
Hippocampal Mossy Fibers
General Neuroscience
Physics
drug effects [Mossy Fibers
Hippocampal]
Glutamate receptor
Brain
Long-term potentiation
Neurochemistry
Neurotransmitters
metabolism [Neurotransmitter Agents]
drug effects [Presynaptic Terminals]
Electrophysiology
metabolism [Presynaptic Terminals]
Mossy Fibers
Hippocampal
Physical Sciences
Thermodynamics
Engineering and Technology
Synaptic Vesicles
Glutamate
Cellular Structures and Organelles
Anatomy
Cellular Types
Function and Dysfunction of the Nervous System
General Agricultural and Biological Sciences
Research Article
QH301-705.5
Presynaptic Terminals
Glutamic Acid
Neurophysiology
Surgical and Invasive Medical Procedures
Biology
Synaptic vesicle
General Biochemistry
Genetics and Molecular Biology
ultrastructure [Mossy Fibers
Hippocampal]
Animals
ddc:610
Active zone
Vesicles
metabolism [Synaptic Vesicles]
General Immunology and Microbiology
Functional Electrical Stimulation
metabolism [Glutamic Acid]
Colforsin
Biology and Life Sciences
Cell Biology
Mice
Inbred C57BL
Microscopy
Fluorescence
Multiphoton
chemistry
Cellular Neuroscience
Synaptic plasticity
Synapses
Biophysics
Neuroscience
Zdroj: PLoS Biology, Vol 19, Iss 6, p e3001149 (2021)
PLoS Biology
PLoS biology 19(6), e3001149 (2021). doi:10.1371/journal.pbio.3001149
ISSN: 1545-7885
1544-9173
DOI: 10.1371/journal.pbio.3001149
Popis: Synaptic plasticity is a cellular model for learning and memory. However, the expression mechanisms underlying presynaptic forms of plasticity are not well understood. Here, we investigate functional and structural correlates of presynaptic potentiation at large hippocampal mossy fiber boutons induced by the adenylyl cyclase activator forskolin. We performed 2-photon imaging of the genetically encoded glutamate sensor iGluu that revealed an increase in the surface area used for glutamate release at potentiated terminals. Time-gated stimulated emission depletion microscopy revealed no change in the coupling distance between P/Q-type calcium channels and release sites mapped by Munc13-1 cluster position. Finally, by high-pressure freezing and transmission electron microscopy analysis, we found a fast remodeling of synaptic ultrastructure at potentiated boutons: Synaptic vesicles dispersed in the terminal and accumulated at the active zones, while active zone density and synaptic complexity increased. We suggest that these rapid and early structural rearrangements might enable long-term increase in synaptic strength.
This study uses several high-resolution imaging techniques to investigate the structural correlates of presynaptic potentiation at hippocampal mossy fiber boutons, observing an increase in release sites and in release synchronicity accompanied by synaptic vesicle dispersion in the terminal and accumulation at release sites, but no modulation of the distance between calcium channel and release sites.
Databáze: OpenAIRE
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