Effect of Sucrose Diet on Insulin Secretion In Vivo and In Vitro and on Triglyceride Storage and Mobilisation of the Heart of Rats
| Autor: | R. Gutman, A. Chicco, Nusimovich B, N. Mocchiutti, Yolanda B. Lombardo, de Rodi Ma |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Sucrose medicine.medical_specialty Lipolysis Endocrinology Diabetes and Metabolism Clinical Biochemistry In Vitro Techniques Biochemistry Glucagon chemistry.chemical_compound Endocrinology Insulin resistance In vivo Internal medicine Insulin Secretion Dietary Carbohydrates medicine Animals Insulin Incubation Triglycerides Glycogen Myocardium Lipid Mobilization Biochemistry (medical) Heart Rats Inbred Strains General Medicine Glucose Tolerance Test medicine.disease Rats chemistry Perfusion Hyperinsulinism |
| Zdroj: | Hormone and Metabolic Research. 15:69-76 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-2007-1018633 |
| Popis: | Basal heart triacylglycerol (TG) (mumole triacylglycerol/g of dry weight) (- before "in vitro" Langendorff perfusion -) was significantly higher in animals rendered chronically hypertriglyceridaemic (H) by a 63% sucrose-rich diet than in controls (C, standard diet); 28 +/- 2.6 means + SEM vs. 19.3 +/- 1.2; respectively (p less than 0.01). After 40' perfusion with Krebs-Henseleit buffer + 5.5 mM glucose, 2.5 mM Ca++, TG content fell to 14.2 +/- 0.6 in C and 14.9 +/- 1.9 in H (n.S.). Administration of 1 n mol x min-1 of glucagon (Gn) from min 20 to 40 reduced TG to 9.0 +/- 0.5 in C (p less than 0.05). In contrast no effect of Gn was observed in H (TG at min 40: 16.7 +/- 2.5). Glycogen (Gly) content (mumol/g of dry weight) after Gn perfusion fell from 30 +/- 1.9 to 17 +/- 2.1 (p less than 0.01) in C, while again no effect was recorded in H. "In vivo" plasma glucose fractional coefficient disappearance rate was lower (p less than 0.001) in H: 1.01 x 10(-2) +/- 0.09 x 10(-2) vs 2.61 x 10(-2) +/- 0.14 x 10(-2) in C, in spite of H showing hyperinsulin secretion. Hyperinsulinism was further documented by "in vitro" Iri release studies from incubated pancreas pieces. In the absence of glucose (G) from the incubation medium H produced 541 +/- 19.8 mU/mg weight Tissue/20', while C produced 91.2 +/- 12.7 (p less than 0.001). With 100 mg% G, H released 1058 +/- 259 and C 377 +/- 82.5 (p less than 0.001). It is suggested that hyperinsulin secretion plus insulin resistance may account for the above findings. |
| Databáze: | OpenAIRE |
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