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The simultaneous detection and quantitative determination of a very large number of metabolites in physiological fluids or tissues, and the subsequent comparison of these data with reference values, is a process which has commonly been called “metabolic profiling” analysis. Profiles generated by gas-liquid chromatography on packed or capillary columns are readily analyzed by mass spectrometry, utilizing reverse library search procedures to identify individual components. Emphasis will be given here to procedures for the pre-purification of organic acids from plasma, to the use of quartz capillaries for metabolic profiling analysis, and to the sensitivity of this method for trace components. |
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