Garcinia kola seeds may prevent cognitive and motor dysfunctions in a type 1 diabetes mellitus rat model partly by mitigating neuroinflammation
| Autor: | Mohammed Farahna, Ahmed El-Tahir, Muaawia A. Hamza, Yahia M. Bushara, Ambrose C Dibia, Sayed Y. Osman, Paul F. Seke Etet, Lorella Vecchio, Gwiria M. H. Satti |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Anti-Inflammatory Agents Garcinia kola Administration Oral Neuroprotection Streptozocin Diabetes Mellitus Experimental 03 medical and health sciences symbols.namesake Cognition 0302 clinical medicine Internal medicine Diabetes mellitus medicine Animals Rats Wistar Type 1 diabetes biology Glial fibrillary acidic protein Plant Extracts business.industry Insulin Streptozotocin medicine.disease biology.organism_classification Rats Surgery Diabetes Mellitus Type 1 Neuroprotective Agents Treatment Outcome 030104 developmental biology Endocrinology Complementary and alternative medicine Motor Skills Seeds Nissl body symbols biology.protein business 030217 neurology & neurosurgery Phytotherapy medicine.drug |
| Zdroj: | Journal of Complementary and Integrative Medicine. 14 |
| ISSN: | 1553-3840 |
| DOI: | 10.1515/jcim-2016-0167 |
| Popis: | Background We reported recently that extracts of seeds of Garcinia kola, a plant with established hypoglycemic properties, prevented the loss of inflammation-sensible neuronal populations like Purkinje cells in a rat model of type 1 diabetes mellitus (T1DM). Here, we assessed G. kola extract ability to prevent the early cognitive and motor dysfunctions observed in this model. Methods Rats made diabetic by single injection of streptozotocin were treated daily with either vehicle solution (diabetic control group), insulin, or G. kola extract from the first to the 6th week post-injection. Then, cognitive and motor functions were assessed using holeboard and vertical pole behavioral tests, and animals were sacrificed. Brains were dissected out, cut, and processed for Nissl staining and immunohistochemistry. Results Hyperglycemia (209.26 %), body weight loss (-12.37 %), and T1DM-like cognitive and motor dysfunctions revealed behavioral tests in diabetic control animals were not observed in insulin and extract-treated animals. Similar, expressions of inflammation markers tumor necrosis factor (TNF), iba1 (CD68), and Glial fibrillary acidic protein (GFAP), as well as decreases of neuronal density in regions involved in cognitive and motor functions (-49.56 % motor cortex, -33.24 % medial septal nucleus, -41.8 % /-37.34 % cerebellar Purkinje /granular cell layers) were observed in diabetic controls but not in animals treated with insulin or G. kola. Conclusions Our results indicate that T1DM-like functional alterations are mediated, at least partly, by neuroinflammation and neuronal loss in this model. The prevention of the development of such alterations by early treatment with G. kola confirms the neuroprotective properties of the plant and warrant further mechanistic studies, considering the potential for human disease. |
| Databáze: | OpenAIRE |
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