The miR-345-3p/PPP2CA signaling axis promotes proliferation and invasion of breast cancer cells

Autor:	Qian Zeng, Fangfang Jin, Husun Qian, Hongling Chen, Yange Wang, Dian Zhang, Yu Wei, Tingmei Chen, Bianqin Guo, Chengsen Chai
Rok vydání:	2021
Předmět:	Cancer Research Carcinogenesis Breast Neoplasms General Medicine Mice MicroRNAs Phosphatidylinositol 3-Kinases Cell Movement Cell Line Tumor Animals Humans Female Neoplasm Invasiveness Protein Phosphatase 2 Proto-Oncogene Proteins c-akt Cell Proliferation Signal Transduction
Zdroj:	Carcinogenesis
ISSN:	1460-2180 0143-3334
DOI:	10.1093/carcin/bgab124
Popis:	Breast cancer is the most common malignancy among women worldwide. Functional studies have demonstrated that miRNA dysregulation in many cases of cancer, in which miRNAs act as either oncogenes or tumor suppressor. Here we report that miR-345-3p is generally upregulated in breast cancer tissues and breast cancer cell lines. Overexpression and inhibition of miR-345-3p revealed its capacity in regulating proliferation and invasion of breast cancer cells. Further research identified protein phosphatase 2 catalytic subunit alpha (PPP2CA), a suppressor of AKT phosphorylation, as a candidate target of miR-345-3p. In vitro, miR-345-3p mimics promoted AKT phosphorylation by targeting its negative regulator, PPP2CA. Blocking miR-345-3p relieved its inhibition of PPP2CA, which attenuated PI3K-AKT signaling pathway. In vivo, inhibiting miR-345-3p by miR-345-3p-inhibition lentivirus suppressed tumor growth and invasiveness in mice. Together, the miR-345-3p/PPP2CA signaling axis exhibits tumor-promoting functions by regulating proliferation and invasion of breast cancer cells. These data provide a clue to novel therapeutic approaches for breast cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::685fc7c60925a378f57251fa90a88207 https://doi.org/10.1093/carcin/bgab124 Zobrazit plný text záznamu