Comparison of endogenous murine leukemia virus proviral organization and RNA expression in 3-methylcholanthrene-induced and spontaneous thymic lymphomas in RF and AKR mice
| Autor: | J Chinsky, Leslie A. Leinwand, M. Jackson, Geoffrey J. Childs, Frank Lilly, M. M. Goodenow |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Genes
Viral Lymphoma Transcription Genetic viruses Immunology Mice Inbred Strains Microbiology Virus Cell Line Nucleic acid thermodynamics chemistry.chemical_compound Mice Mice Inbred AKR Virology hemic and lymphatic diseases Murine leukemia virus Animals Gene Leukemia Experimental biology Base Sequence RNA Nucleic Acid Hybridization DNA Restriction Enzymes Thymus Neoplasms biochemical phenomena metabolism and nutrition biology.organism_classification Long terminal repeat AKR murine leukemia virus Leukemia Virus Murine chemistry Insect Science Female DNA Methylcholanthrene Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-538X |
| Popis: | 3-Methylcholanthrene-induced T-cell thymic lymphomas in RF mice were examined for involvement of murine leukemia virus (MuLV)-related sequences in leukemogenesis. Both the expression of MuLV-related RNA species and the organization of endogenous MuLV proviral DNA were analyzed. Of 27 primary tumors examined, only 5 exhibited elevated MuLV-related RNA species homologous to xenotropic specific env DNA. None of these RNA species hybridized with ecotropic p15E DNA sequences. Only two of these five tumors contained MuLV-like RNA species that hybridized with ecotropic MuLV long terminal repeat sequences, despite the probe's ability to detect both ecotropic MuLV and mink cell focus-inducing viral RNA. No muLV resembling mink cell focus-inducing virus whose expression could be correlated with lymphomagenesis was detected in either preleukemic thymocytes, primary 3-methylcholanthrene-induced thymic tumors, tumors passaged in vivo, or cell lines derived from tumors. Restriction endonuclease analysis of DNA from both primary tumors and cell lines failed to reveal either proviral DNA with recombinant env genes or rearrangement of endogenous MuLV proviruses. Therefore, chemically induced lymphomagenesis in RF mice appears different from the spontaneous lymphomagenic process in AKR mice with respect to the involvement of endogenous MuLV sequences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|