Fluoride flux in the rabbit CCD: A pH-dependent event
| Autor: | Harold T. Campbell, Gary M. Whitford, Alexander J. Rouch |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
4-Acetamido-4'-isothiocyanatostilbene-2 2'-disulfonic Acid Kidney Cortex Renal cortex Ph dependent 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid In Vitro Techniques Chloride Epithelium Excretion Fluorides chemistry.chemical_compound Chlorides Internal medicine medicine Animals Kidney Tubules Collecting Lagomorpha biology Radiochemistry Hydrogen-Ion Concentration biology.organism_classification Acetazolamide Electrophysiology Endocrinology medicine.anatomical_structure chemistry Nephrology DIDS Rabbits Fluoride medicine.drug |
| Zdroj: | Kidney International. 41:342-349 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1992.47 |
| Popis: | Fluoride flux in the rabbit CCD: A pH-dependent event. We measured fluoride flux (JF; pmol · min-1 · mm-1) in the isolated rabbit cortical collecting duct (CCD) to investigate the determining factors of JF. The perfusate contained 100 µM fluoride and the bath was fluoride-free. Osmotically-induced lumen-to-bath water flux did not affect JF. When perfusate pH was reduced from 7.4 to 6.1 and from 6.1 to 5.0, JF increased from 0.008 ± 0.002 to 0.027 ± 0.007 (P < 0.01) and from 0.018 ± 0.003 to 0.040 ± 0.005 (P < 0.01), respectively. Acetazolamide at 10-4M in the bath reduced JF slightly though not statistically. The anion-transport inhibitor, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS), at 10-4M in the perfusate did not affect JF. Substitution of luminal chloride with gluconate failed to affect JF in tubules from normal rabbits or from rabbits treated with deoxycorticosterone which stimulates chloride-bicarbonate exchange in the CCD. JF showed no correlation with transepithelial voltage which ranged from +4 to -104 mV. We conclude that the luminal pH represents the primary determining factor influencing JF in the rabbit CCD, and fluoride does not use a chloride-mediated or a DIDS-inhibitory transport pathway. |
| Databáze: | OpenAIRE |
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