Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer
| Autor: | Junming Bi, Wei Dong, Jinli Han, Jian Huang, Meihua Yang, Hongwei Liu, Dongliang Chen, Tianxin Lin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Aging B4GALT3 Mice Nude circ0001380 Biology Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Cell Movement Circular RNA In vivo Cell Line Tumor medicine Animals Humans Gene silencing Neoplasm Invasiveness Viability assay Gene Cell Proliferation Mice Inbred BALB C Cell growth RNA Cancer circular RNA RNA Circular Cell Biology Middle Aged Galactosyltransferases medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Urinary Bladder Neoplasms 030220 oncology & carcinogenesis miR-1247-3p Cancer research bladder cancer Female circUBXN7 Research Paper Signal Transduction |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.101573 |
| Popis: | Circular RNAs (circRNAs) have recently been confirmed to participate in different pathological processes, including cancer progression. However, the role and precise mechanism of action of the majority of circRNAs have not been elucidated in bladder cancer (BC). Here, we identified a novel circular RNA, termed circUBXN7, which was significantly downregulated in BC tissues compared with matched nontumor tissues. Importantly, we found that decreased circUBXN7 expression was associated with pathological stage, grade and poor prognosis of BC patients. Functional experiments showed that circUBXN7 overexpression dramatically inhibited proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Mechanistically, circUBXN7 could directly bind to miR-1247-3p and reverse the oncogenic effects induced by miR-1247-3p. Furthermore, B4GALT3 was predicted and confirmed to be a target of miR-1247-3p. Rescue experiments demonstrated that circUBXN7 abrogated miR-1247-3p-mediated inhibition of B4GALT3 expression. Finally, silencing of B4GALT3 promoted proliferation and invasion of BC cells; and partially abolished the tumor suppressive effects caused by circUBXN7. Taken together, our study revealed that circUBXN7 serves as a competitive endogenous RNA of miR-1247-3p to elevate B4GALT3 expression, consequently inhibiting cell viability and invasion in BC. The circUBXN7-miR-1247-3p-B4GALT3 regulatory network may provide a new perspective for gene-based treatment strategies for BC. |
| Databáze: | OpenAIRE |
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