Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
| Autor: | Arnold Garbiec, Jolanta Saczko, Zofia Marchewka, Agnieszka Piwowar, Nina Rembiałkowska, Agnieszka Dobosz, Anna Rorbach-Dolata, Sylwester Ślusarczyk, Adam Matkowski, Anna Długosz |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
PC12 cell line
Pharmacology Article Catalysis mangiferin 3-nitropropionic acid Inorganic Chemistry lcsh:Chemistry chemistry.chemical_compound Anemarrhena asphodeloides Xanthone Viability assay Physical and Theoretical Chemistry Cytotoxicity Mangiferin Molecular Biology lcsh:QH301-705.5 Spectroscopy biology Chemistry Organic Chemistry PC12 cells General Medicine biology.organism_classification Computer Science Applications Rhizome lcsh:Biology (General) lcsh:QD1-999 Polyphenol cytotoxicity |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 2510, p 2510 (2020) International Journal of Molecular Sciences Volume 21 Issue 7 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line exposed to the neurotoxic agent&mdash 3-nitropropionic acid (3-NP). The xanthone-enriched fraction of the ethanolic extract of A. asphodeloides (abbreviated from now on as XF, for the Xanthone Fraction), rich in polyphenolic xanthone glycosides, in concentrations from 5 to 100 &mu g/mL, and 3-NP in concentrations from 2.5 to 15 mM, were examined. After 8, 16, 24, 48, and 72 h of exposure of cells to various combinations of 3-NP and XF, the MTT viability assay was performed and morphological changes were estimated by confocal fluorescence microscopy. The obtained results showed a significant increase in the number of cells surviving after treatment with XF with exposure to neurotoxic 3-NP and decreased morphological changes in PC12 cells in a dose and time dependent manner. The most effective protective action was observed when PC12 cells were pre-incubated with the XF. This effect may contribute to the traditional indications of this herb for neurological and cognitive complaints. However, a significant cytotoxicity observed at higher XF concentrations (over 10 µ g/mL) and longer incubation time (48 h) requires caution in future research and thorough investigation into potential adverse effects. |
| Databáze: | OpenAIRE |
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