Endoplasmic reticulum stress induces 5-fluorouracil resistance in human colon cancer cells

Autor: Yong Joo Jeong, Seung Uk Jeong, Soo-Young Na, Sun Jin Boo, Yea Seong Ryu, Min Chang Oh, Xia Han, Mei Jing Piao, Joon Ki Kim, Jin Won Hyun, Kristina Shilnikova, Pattage Madushan Dilhara Jayatissa Fernando, Kyoung Ah Kang, Jeong Eon Park
Rok vydání: 2016
Předmět:
0301 basic medicine
MAPK/ERK pathway
Antimetabolites
Antineoplastic
medicine.medical_specialty
Cell Survival
Colorectal cancer
Health
Toxicology and Mutagenesis
Mitochondrion
Toxicology
03 medical and health sciences
0302 clinical medicine
Cell Line
Tumor
Internal medicine
medicine
Humans
RNA
Small Interfering
Extracellular Signal-Regulated MAP Kinases
Endoplasmic Reticulum Chaperone BiP
Protein kinase B
Heat-Shock Proteins
Pharmacology
ATF6
business.industry
Endoplasmic reticulum
General Medicine
Transfection
Endoplasmic Reticulum Stress
medicine.disease
Activating Transcription Factor 6
Mitochondria
030104 developmental biology
Endocrinology
Drug Resistance
Neoplasm
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Colonic Neoplasms
Unfolded protein response
Cancer research
Calcium
Fluorouracil
business
Proto-Oncogene Proteins c-akt
Zdroj: Environmental Toxicology and Pharmacology. 44:128-133
ISSN: 1382-6689
Popis: Colon cancer can be treated with 5-fluorouracil (5-FU), but 5-FU resistance frequently occurs. We determined whether 5-FU resistance arises as a result of endoplasmic reticulum (ER) stress. 5-FU-resistant SNUC5 colon cancer cells (SNUC5/FUR cells) expressed higher levels of ER stress-related proteins than drug-sensitive SNUC5 cells. SNUC5/FUR cells also exhibited more intense ER staining and higher level of mitochondrial Ca(2+) overload. SNUC5/FUR cells transfected with siRNA against GRP78, ATF6, ERK, or AKT were more sensitive to 5-FU than siControl RNA-transfected cells. These results suggested that 5-FU resistance was associated with ER stress in colon cancer.
Databáze: OpenAIRE
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