| Popis: |
The production of the carrot phytoalexin, 6-methoxymellein, and its immediate biosynthetic precursor, 6-hydroxymellein, was induced in suspension cultures by biotic elicitors (Aspergillus niger pectinase and purified endo-polygalacturonase from Fusarium moniliforme) and by the abiotic elicitor HgCl2. Aspergillus niger pectinase and HgCl2 elicited intracellular accumulation of 6-hydroxymellein, whereas Fusarium moniliforme endo-polygalacturonase induced extracellular 6-methoxymellein production in the culture medium. Culture conditions such as medium composition and 2,4-dichlorophenoxy acetic acid (2,4-D) concentration influenced the amounts of both dihydroisocoumarins synthetized. Moreover, elicitors were phytotoxic and their addition to carrot cell suspension cultures caused an increase of extracellular pH and medium conductivity. |
