Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig
| Autor: | Guo Xiaohong, Wanfeng Zhang, Cao Guoqing, Gao Pengfei, Meng Li, Baoyu Le, Li Bugao, Pengkang Song, Cheng Zhimin, Zhang Ningfang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Candidate gene
lcsh:Animal biochemistry RNA-Seq Biology Article Transcriptome chemistry.chemical_compound KEGG Gene lcsh:QP501-801 lcsh:SF1-1100 Genetics Pig Molecular Mechanism Fatty acid metabolism 0402 animal and dairy science 04 agricultural and veterinary sciences Malate dehydrogenase 1 Pyruvate dehydrogenase complex Animal Breeding and Genetics 040201 dairy & animal science chemistry Meat Quality Candidate Gene Animal Science and Zoology lcsh:Animal culture Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 32, Iss 8, Pp 1084-1094 (2019) Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | OBJECTIVE The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. METHODS The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). RESULTS The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. CONCLUSION Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs. |
| Databáze: | OpenAIRE |
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