The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
| Autor: | Gunter Meister, Witold Filipowicz, Ragna Sack, Inga Loedige, Dimos Gaidatzis |
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| Rok vydání: | 2012 |
| Předmět: |
RNA Stability
Repressor RNA-binding protein Biology Retinoblastoma-like protein 1 Mice hemic and lymphatic diseases microRNA Genetics Protein biosynthesis Animals Humans Point Mutation RNA Messenger Transcription factor Psychological repression Embryonic Stem Cells Messenger RNA RNA-Binding Proteins Molecular biology Protein Structure Tertiary MicroRNAs HEK293 Cells Ribonucleoproteins Protein Biosynthesis RNA Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gks1032 |
| Popis: | TRIM-NHL proteins are conserved regulators of development and differentiation but their molecular function has remained largely elusive. Here, we report an as yet unrecognized activity for the mammalian TRIM-NHL protein TRIM71 as a repressor of mRNAs. We show that TRIM71 is associated with mRNAs and that it promotes translational repression and mRNA decay. We have identified Rbl1 and Rbl2, two transcription factors whose down-regulation is important for stem cell function, as TRIM71 targets in mouse embryonic stem cells. Furthermore, one of the defining features of TRIM-NHL proteins, the NHL domain, is necessary and sufficient to target TRIM71 to RNA, while the RING domain that confers ubiquitin ligase activity is dispensable for repression. Our results reveal strong similarities between TRIM71 and Drosophila BRAT, the best-studied TRIM-NHL protein and a well-documented translational repressor, suggesting that BRAT and TRIM71 are part of a family of mRNA repressors regulating proliferation and differentiation. |
| Databáze: | OpenAIRE |
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