Comparison of different signal peptides for secretion of heterologous proteins in fission yeast
| Autor: | Søren Kjærulff, Martin Roland Jensen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Signal peptide
Recombinant Fusion Proteins Saccharomyces cerevisiae Genetic Vectors Green Fluorescent Proteins Biophysics Cathepsin A Protein Sorting Signals Biochemistry Green fluorescent protein Fungal Proteins Schizosaccharomyces Humans Secretion URA3 Molecular Biology Secretory pathway biology Tumor Necrosis Factor-alpha Cell Biology biology.organism_classification Molecular biology Yeast Cell biology Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 336(3) |
| ISSN: | 0006-291X |
| Popis: | In the fission yeast Schizosaccharomyces pombe, there are relatively few signal peptides available and most reports of their activity have not been comparative. Using sequence information from the S. pombe genome database we have identified three putative signal peptides, designated Cpy, Amy and Dpp, and compared their ability to support secretion of green fluorescent protein (GFP). In the comparison we also included the two well-described secretion signals derived from the precursors of, respectively, the Saccharomyces cerevisiae alpha-factor and the S. pombe P-factor. The capability of the tested signal peptides to direct secretion of GFP varied greatly. The alpha-factor signal did not confer secretion to GFP and all the produced GFP was trapped intracellular. In contrast, the Cpy signal peptide supported efficient secretion of GFP with yields approximating 10 mg/L. We also found that the use of an attenuated version of the S. cerevisiae URA3 marker substantially increases vector copy number and expression yield in fission yeast. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect