Identification of Heat Shock Transcription Factor Genes Involved in Thermotolerance of Octoploid Cultivated Strawberry
| Autor: | Lee-Fong Lin, Chun-Chung Wang, Ming-Lun Chou, Jing-Lian Jheng, Jui-Hung Yang, Wan-Yu Liao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine octoploid cultivated strawberry (Fragaria × ananassa Duch. cv. Toyonoka) Hot Temperature Arabidopsis 01 natural sciences lcsh:Chemistry Transcriptome heat stress Heat Shock Transcription Factors Gene Expression Regulation Plant heat shock transcription factor Illumina sequencing transcriptome thermotolerance lcsh:QH301-705.5 Spectroscopy Heat-Shock Proteins Genetics General Medicine Fragaria Plants Genetically Modified Computer Science Applications DNA-Binding Proteins Thermotolerance Recombinant Fusion Proteins Biology Catalysis Article Inorganic Chemistry 03 medical and health sciences Stress Physiological Amino Acid Sequence Physical and Theoretical Chemistry Molecular Biology Gene Transcription factor Illumina dye sequencing Organic Chemistry biology.organism_classification Fusion protein Heat shock factor 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Sequence Alignment Heat-Shock Response 010606 plant biology & botany Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 17, Iss 12, p 2130 (2016) International Journal of Molecular Sciences; Volume 17; Issue 12; Pages: 2130 |
| ISSN: | 1422-0067 |
| Popis: | Heat shock transcription factors (HSFs) are mainly involved in the activation of genes in response to heat stress as well as other abiotic and biotic stresses. The growth, development, reproduction, and yield of strawberry are strongly limited by extreme temperatures and droughts. In this study, we used Illumina sequencing and obtained transcriptome data set from Fragaria × ananassa Duchessne cv. Toyonoka. Six contigs and three unigenes were confirmed to encode HSF proteins (FaTHSFs). Subsequently, we characterized the biological functions of two particularly selected unigenes, FaTHSFA2a and FaTHSFB1a, which were classified into class A2 and B HSFs, respectively. Expression assays revealed that FaTHSFA2a and FaTHSFB1a expression was induced by heat shock and correlated well with elevated ambient temperatures. Overexpression of FaTHSFA2a and FaTHSFB1a resulted in the activation of their downstream stress-associated genes, and notably enhanced the thermotolerance of transgenic Arabidopsis plants. Besides, both FaTHSFA2a and FaTHSFB1a fusion proteins localized in the nucleus, indicating their similar subcellular distributions as transcription factors. Our yeast one-hybrid assay suggested that FaTHSFA2a has trans-activation activity, whereas FaTHSFB1a expresses trans-repression function. Altogether, our annotated transcriptome sequences provide a beneficial resource for identifying most genes expressed in octoploid strawberry. Furthermore, HSF studies revealed the possible insights into the molecular mechanisms of thermotolerance, thus rendering valuable molecular breeding to improve the tolerance of strawberry in response to high-temperature stress. |
| Databáze: | OpenAIRE |
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