Dynamics of the HP1β-PCNA-containing complexes in DNA replication and repair
| Autor: | Dominika O. Trembecka-Lucas, Aleksander T. Szczurek, Jurek W. Dobrucki |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
DNA Repair Chromosomal Proteins Non-Histone DNA repair Eukaryotic DNA replication DNA replication protein interactions DNA polymerase delta DNA replication factor CDT1 Replication factor C Control of chromosome duplication Proliferating Cell Nuclear Antigen HP1 beta Fluorescence Resonance Energy Transfer PCNA Humans FRET-FLIM Replication protein A Cell Nucleus biology DNA Cell Biology Molecular biology Proliferating cell nuclear antigen Cell biology Chromatin Assembly Factor-1 Protein Subunits Chromobox Protein Homolog 5 FRAP biology.protein Origin recognition complex Dimerization Research Paper HeLa Cells Protein Binding Transcription Factors |
| Zdroj: | Nucleus. 4:74-82 |
| ISSN: | 1949-1042 1949-1034 |
| DOI: | 10.4161/nucl.23683 |
| Popis: | Heterochromatin protein 1 (HP1), a small non-histone chromosomal protein, was recently shown to form a complex in vivo with Proliferating Cell Nuclear Antigen (PCNA), a key factor in DNA replication. The complex, which requires HP1β in a form of a dimer, is engaged in DNA repair and replication. We now provide further evidence based on FRET-FLIM live cell studies confirming the association and close proximity between HP1β and PCNA in the complex. We also demonstrate using FRAP, that although HP1β-PCNA complexes are highly mobile in nonreplicating nuclei, when engaged in DNA replication, they become bound and do not exchange with the mobile pool. These observations are in agreement with a notion that a subpopulation of HP1 molecules interact with PCNA in vivo during DNA replication. Similarly, HP1β which is associated with PCNA in regions of DNA repair, is bound and does not exchange with the mobile pool, suggesting that HP1β in association with PCNA may be a component of a DNA repair complex. |
| Databáze: | OpenAIRE |
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