The activity of 1-naphthol-UDP-glucuronosyltransferase in the brain
| Autor: | Jean-François Ghersi-Egea, Alain Minn, Gérard Siest, D. Decolin, B. Walther |
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| Rok vydání: | 1987 |
| Předmět: |
Male
medicine.medical_specialty Guinea Pigs Rats Gunn Glucuronidation Naphthols Cellular and Molecular Neuroscience chemistry.chemical_compound Species Specificity Microsomes Internal medicine medicine Animals Glucuronosyltransferase Enzyme inducer Pharmacology biology Brain Rats Inbred Strains Rats carbohydrates (lipids) Kinetics Uridine diphosphate Endocrinology Digitonin Biochemistry chemistry biology.protein Microsome Phenobarbital Rabbits Glucuronide Drug metabolism medicine.drug |
| Zdroj: | Neuropharmacology. 26:367-372 |
| ISSN: | 0028-3908 |
| DOI: | 10.1016/0028-3908(87)90190-0 |
| Popis: | Cerebral microsomes catalysed efficiently the glucuronidation of 1-naphthol, this formation of glucuronide being activated by treatment with Triton X-100 or digitonin. Activated microsomes from the brain of the rat conjugated 1-naphthol with an apparent Km of 95 microM and a Vmax of 5.47 nmol/hr mg protein at 30 degrees C. Microsomal uridine diphosphate (UDP)-glucuronosyltransferase activity in brain towards 1-naphthol was not significantly induced by pretreatment of animals with 3-methylcholanthrene or phenobarbital. These data suggest that UDP-glucuronosyltransferases in brain are different from the hepatic enzymes with regard to biochemical parameters and in response to inducers of drug metabolism. The hepatic UDP-glucuronosyltransferase deficiency in Gunn rats was also observed in the brain. |
| Databáze: | OpenAIRE |
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