Microtiter Screening Reveals Oxygen-Dependent Antimicrobial Activity of Natural Products Against Mastitis-Causing Bacteria
| Autor: | Michelle McConnell, David Rennison, Ayana Menorca, Essie M. Van Zuylen, Scott A. Ferguson, Chen-Yi Cheung, Adam Heikal, Gregory M. Cook, Kip Bodle, Scott McDougall, Margaret A. Brimble |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus natural product inhibitors lcsh:QR1-502 medicine.disease_cause mastitis Microbiology lcsh:Microbiology 03 medical and health sciences Minimum inhibitory concentration medicine 030304 developmental biology Original Research Streptococcus uberis chemistry.chemical_classification 0303 health sciences Reactive oxygen species Minimum bactericidal concentration biology 030306 microbiology Chemistry β-lapachone Antimicrobial biology.organism_classification medicine.disease Mastitis antimicrobial oxygen-dependent Bacteria |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) Frontiers in Microbiology |
| Popis: | In this study we investigated the influence of oxygen availability on a phenotypic microtiter screen to identify new, natural product inhibitors of growth for the bovine mastitis-causing microorganisms; Streptococcus uberis, Staphylococcus aureus, and Escherichia coli. Mastitis is a common disease in dairy cattle worldwide and is a major cause of reduced milk yield and antibiotic usage in dairy herds. Prevention of bovine mastitis commonly relies on the application of teat disinfectants that contain either iodine or chlorhexidine. These compounds are used extensively in human clinical settings and increased tolerance to chlorhexidine has been reported in both Gram-positive and Gram-negative microorganisms. As such new, non-human use alternatives are required for the agricultural industry. Our screening was conducted under normoxic (20% oxygen) and hypoxic ( |
| Databáze: | OpenAIRE |
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