Mrpl10 and Tbp Are Suitable Reference Genes for Peripheral Nerve Crush Injury

Autor: Qianqian Shan, Yaxian Wang, Yali Meng, Sheng Yi, Jiacheng Pan
Rok vydání: 2017
Předmět:
0301 basic medicine
geNorm
lcsh:Chemistry
Rats
Sprague-Dawley
Peripheral Nerve Injuries
Ganglia
Spinal
Reference genes
Ubiquitin C
Phosphoglycerate kinase 1
lcsh:QH301-705.5
Spectroscopy
housekeeping gene
Genetics
education.field_of_study
Genes
Essential
NormFinder
General Medicine
Reference Standards
Sciatic nerve injury
Immunohistochemistry
Sciatic Nerve
Computer Science Applications
Housekeeping gene
Peripheral nerve injury
Female
Sciatic nerve
medicine.symptom
Ribosomal Proteins
dorsal root ganglion
Nerve Crush
Biology
Article
Catalysis
Inorganic Chemistry
Crush Injuries
03 medical and health sciences
medicine
Animals
Physical and Theoretical Chemistry
education
Molecular Biology
rat peripheral nerve injury
distal nerve stump
Organic Chemistry
Nerve injury
TATA-Box Binding Protein
medicine.disease
Molecular biology
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Software
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 263
International Journal of Molecular Sciences, Vol 18, Iss 2, p 263 (2017)
ISSN: 1422-0067
DOI: 10.3390/ijms18020263
Popis: Peripheral nerve injury triggers the dysregulation of a large number of genes at multiple sites, including neurons, peripheral nerve stump, and the target organ. Housekeeping genes were frequently used as reference genes to normalize the expression values of target genes. Suitable selection of housekeeping genes that are stably expressed after nerve injury minimizes bias elicited by reference genes and thus helps to better and more sensitively reflect gene expression changes. However, many housekeeping genes have been used as reference genes without testing the expression patterns of themselves. In the current study, we calculated the expression stability of nine commonly used housekeeping genes, such as 18S (18S ribosomal RNA), Actb (β-actin), CypA (cyclophilin A), Gapdh (glyceraldehydes-3-phosphate dehydrogenase), Hprt (hypoxanthine guanine phosphoribosyl transferase), Pgk1 (phosphoglycerate kinase 1), Tbp (TATA box binding protein), Ubc (ubiquitin C), YwhaZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation), and four newly identified housekeeping genes, including Ankrd27 (Ankyrin repeat domain 27), Mrpl10 (mitochondrial ribosomal protein L10), Rictor (rapamycin-insensitive companion of mTOR, Complex 2), and Ubxn 11 (UBX domain protein 11), in both distal sciatic nerve samples and dorsal root ganglion (DRG) samples after sciatic nerve injury. Our results suggested that following peripheral nerve injury, Mrpl10 and Tbp might be used as suitable reference genes for sciatic nerve stump and DRGs, respectively.
Databáze: OpenAIRE
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