Multiphasic Approach Reveals Genetic Diversity of Environmental and Patient Isolates of Mycobacterium mucogenicum and Mycobacterium phocaicum Associated with an Outbreak of Bacteremias at a Texas Hospital
| Autor: | Roger E. Morey, Denise Dunbar, Nadege Charles, Marcella Kuan, Arjun Srinivasan, Vickie Bennett, Mitchell A. Yakrus, Margaret M. Williams, W. Ray Butler, Michael A. Jhung, Kenneth C. Jost, Bette Jensen, Sean R. Toney, Toïdi Adékambi, Robert C. Cooksey, Glenn P. Morlock, Alicia M. Shams |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Male Chaperonins Genotype Molecular Sequence Data Bacteremia Public Health Microbiology DNA Ribosomal Polymerase Chain Reaction Applied Microbiology and Biotechnology Disease Outbreaks Mycobacterium Restriction fragment Microbiology Bacterial Proteins RNA Ribosomal 16S Environmental Microbiology Pulsed-field gel electrophoresis Cluster Analysis Humans Mycobacterium mucogenicum Aged Genetics Cross Infection Molecular Epidemiology Mycobacterium Infections Ecology Molecular epidemiology biology Genetic Variation Chaperonin 60 DNA-Directed RNA Polymerases Sequence Analysis DNA rpoB biology.organism_classification DNA Fingerprinting Texas Hospitals Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Random Amplified Polymorphic DNA Technique DNA profiling Mycobacterium phocaicum biology.protein Female Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 74:2480-2487 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Between March and May 2006, a Texas hospital identified five Mycobacterium mucogenicum bloodstream infections among hospitalized oncology patients using fluorescence high-performance liquid chromatography analysis of mycolic acids. Isolates from blood cultures were compared to 16 isolates from environmental sites or water associated with this ward. These isolates were further characterized by hsp65 , 16S rRNA, and rpoB gene sequencing, hsp65 PCR restriction analysis, and molecular typing methods, including repetitive element PCR, random amplified polymorphic DNA PCR, and pulsed-field gel electrophoresis (PFGE) of large restriction fragments. Three of five patient isolates were confirmed as M. mucogenicum and were in a single cluster as determined by all identification and typing methods. The remaining two patient isolates were identified as different strains of Mycobacterium phocaicum by rpoB sequence analysis. One of these matched an environmental isolate from a swab of a hand shower in the patient's room, while none of the clinical isolates of M. mucogenicum matched environmental strains. Among the other 15 environmental isolates, 11 were identified as M. mucogenicum and 4 as M. phocaicum strains, all of which were unrelated by typing methods. Although the 16S rRNA gene sequences matched for all 14 M. mucogenicum isolates, there were two each of the hsp65 and rpoB sequevars, seven PCR typing patterns, and 12 PFGE patterns. Among the seven M. phocaicum isolates were three 16S rRNA sequevars, two hsp65 sequevars, two rpoB sequevars, six PCR typing patterns, and six PFGE patterns. This outbreak represents the first case of catheter-associated bacteremia caused by M. phocaicum and the first report of clinical isolates from a U.S. hospital. The investigation highlights important differences in the available typing methods for mycobacteria and demonstrates the genetic diversity of these organisms even within narrow confines of time and space. |
| Databáze: | OpenAIRE |
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