Emergence of Multiple Cytomegalovirus Strains in Blood and Lung of Lung Transplant Recipients
| Autor: | Alexander Zoufaly, Therese Popow-Kraupp, Elisabeth Puchhammer-Stöckl, Irene Görzer, Peter Jaksch, C. Bauer, Walter Klepetko |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Human cytomegalovirus Time Factors Adolescent Opportunistic infection Cytomegalovirus Opportunistic Infections Biology medicine.disease_cause Antiviral Agents Polymerase Chain Reaction Betaherpesvirinae medicine Humans Viremia Lung Phylogeny Aged Transplantation Base Sequence virus diseases Middle Aged biology.organism_classification medicine.disease Virology medicine.anatomical_structure Cytomegalovirus Infections DNA Viral Immunology Female Viral disease Serostatus Bronchoalveolar Lavage Fluid Polymorphism Restriction Fragment Length Lung Transplantation |
| Zdroj: | Transplantation. 81:187-194 |
| ISSN: | 0041-1337 |
| Popis: | Background. Cytomegalovirus (CMV) is a major pathogen in lung transplant recipients (LTRs). The emergence of different CMV strains in lung and blood after transplantation has not yet been analyzed. Methods. In total, 75 serum and 91 broncheoalveolar lavage (BAL) samples obtained from 25 LTRs in the follow-up after transplantation were tested for the presence of different CMV strains. The gB, gN, and gO genes of the CMV isolates were analyzed by subtype-specific PCR, restriction fragment length polymorphism (RFLP), sequencing, and phylogenetic analysis. Results. Mixed CMV-strain populations were detected after cessation of antiviral prophylaxis in up to 80% and 90% of the patients' BAL and serum, respectively, and this was independent of the CMV serostatus of donor and recipient. In five patients, the same single CMV strain was consistently detectable over at least 1 year in lung and blood, although in two of these cases donor and recipient had both been CMV-seropositive. Most CMV strains were distributed in the lung and blood compartment. Symptomatic CMV infection within the first year after transplantation was observed only in patients with mixed CMV-strain populations (P |
| Databáze: | OpenAIRE |
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