Network activity-independent coordinated gene expression program for synapse assembly
| Autor: | Christopher N. G. Anderson, Seth G. N. Grant, Luis M. Valor, Paul Charlesworth, Lawrence Humphreys |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Time Factors Transcription Genetic Synaptogenesis Biology Hippocampus Models Biological Synapse Mice Gene expression Animals Gene Cells Cultured Oligonucleotide Array Sequence Analysis Neurons Genetics Regulation of gene expression Genome Multidisciplinary Synapse assembly Gene Expression Profiling Genomics Biological Sciences Cell biology Gene expression profiling Gene Expression Regulation Synapses |
| Zdroj: | Proceedings of the National Academy of Sciences. 104:4658-4663 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Global biological datasets generated by genomics, transcriptomics, and proteomics provide new approaches to understanding the relationship between the genome and the synapse. Combined transcriptome analysis and multielectrode recordings of neuronal network activity were used in mouse embryonic primary neuronal cultures to examine synapse formation and activity-dependent gene regulation. Evidence for a coordinated gene expression program for assembly of synapses was observed in the expression of 642 genes encoding postsynaptic and plasticity proteins. This synaptogenesis gene expression program preceded protein expression of synapse markers and onset of spiking activity. Continued expression was followed by maturation of morphology and electrical neuronal networks, which was then followed by the expression of activity-dependent genes. Thus, two distinct sequentially active gene expression programs underlie the genomic programs of synapse function. |
| Databáze: | OpenAIRE |
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