Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
| Autor: | M. Hoeneisen, Ana S. Cifuentes, Silvia Vargas, Nelson González, Mariela A. González |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
carotenogenesis
Pluvialis growth Xanthophylls General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Dry weight Astaxanthin Chlorophyta Botany Food science Biomass Phototropism Carotenoid lcsh:QH301-705.5 Chromatography High Pressure Liquid chemistry.chemical_classification Haematococcus pluvialis biology General Medicine biology.organism_classification beta Carotene astaxanthin chemistry lcsh:Biology (General) Spectrophotometry Xanthophyll Urea General Agricultural and Biological Sciences Sodium acetate Biotechnology |
| Zdroj: | Biological Research, Vol 36, Iss 3-4, Pp 343-357 (2003) Biological Research v.36 n.3-4 2003 SciELO Chile CONICYT Chile instacron:CONICYT Biological Research, Volume: 36, Issue: 3-4, Pages: 343-357, Published: 2003 |
| ISSN: | 0717-6287 0716-9760 |
| Popis: | The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 mumol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8%), 2) N-deprivation and 3) high PFD (150 mumol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k = 0.7 div day-1, 228-258 mg l-1 and 2.0 x 10(5)-2.5 x 10(5) cells ml-1 at both PFD, respectively). With increasing acetate concentration, a slight increment in growth occurred only at 85 mumol m-2 s-1. Light was the best inductive carotenogenic factor, and the highest carotenoid production (4.9 mg l-1, 25.0 pg cell-1) was obtained in cultures pre-grown in nitrate at low light. The NaCl caused an increase in carotenoid content per cell at increasing salt concentrations, but resulted in a high cell mortality and did not produce any increment in carotenoid content per volume compared to cultures grown at 150 mumol m-2 s-1. The highest carotenoid content per cell (22 pg) and astaxanthin content per dry weight (10.3 mg g-1) (1% w/w) were obtained at 85 mumol m-2 s-1 with 0.8% NaCl. |
| Databáze: | OpenAIRE |
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