Comparison Of The Effect Of Cytochalasin B And Paclitaxel (Taxol Tm) On Cryopreservation Of Icr Mouse Oocytes
| Autor: | Shao-Ping Weng, Po-Ning Lee, I-Chuan Chuang, Yiu-Tai Li, Ching-Huan Tsai, Cheng-Tsung Kuo |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty Pregnant Mare Serum Gonadotropin Biology lcsh:Gynecology and obstetrics Cryopreservation Human chorionic gonadotropin Andrology paclitaxel chemistry.chemical_compound Human fertilization Obstetrics and Gynaecology medicine oocyte Survival rate Cytochalasin B lcsh:RG1-991 mouse Gynecology cytochalasin B cumulus cell Obstetrics and Gynecology Embryo Oocyte vitrification medicine.anatomical_structure chemistry |
| Zdroj: | Taiwanese Journal of Obstetrics & Gynecology, Vol 44, Iss 1, Pp 48-51 (2005) |
| ISSN: | 1028-4559 |
| DOI: | 10.1016/s1028-4559(09)60106-6 |
| Popis: | Summary Objective To investigate the benefits of the cytoskeleton stabilizers paclitaxel and cytochalasin B (CCB) in the oocyte vitrification procedure. Materials and Methods ICR mice aged 4-6 weeks were superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin. In Experiment 1, all retrieved eggs were randomly assigned to group A or B for the cumulus cell effect test and went through the vitrification protocol. In Experiment 2, the oocytes were allocated randomly to one of two subgroups for the cytoskeleton stabilizer tests: subgroup B, paclitaxel, and subgroup C, CCB. Group B in Experiment 1 served as the control (i.e. subgroup A) in Experiment 2. Results In Experiment 1, denuded oocytes had a higher fertilization rate after thawing than cumulus-enclosed ones. Accordingly, cumulus-free oocytes were chosen for Experiment 2. In Experiment 2, there were significant differences in post-thaw survival rates and fertilization rates between subgroups A and B. There was also a significant difference in fertilization rates between subgroups A and C. However, there were no differences in cleavage rates of 2-4- and 6-8-cell embryos between the subgroups. Conclusion Cumulus-free oocytes have greater developmental competence than cumulus-enclosed ones. Adding paclitaxel significantly improves the survival rate and fertilization rates of post-thaw cumulus-free ICR mouse oocytes. The addition of CCB also improves the fertilization rate of post-thaw oocytes. |
| Databáze: | OpenAIRE |
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