Transcriptome Profiling of the Retained Fetal Membranes—An Insight in the Possible Pathogenesis of the Disease
Autor: | Katarzyna Ropka-Molik, Tomasz Szmatoła, Ilona Kowalczyk-Zieba, Marta J. Siemieniuch, Katarzyna Piórkowska, Izabela Woclawek-Potocka, Joanna Jaworska |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
placenta Biology Endometrium Article Pathogenesis Transcriptome reproduction 03 medical and health sciences Retained placenta Placenta lcsh:Zoology medicine lcsh:QL1-991 Gene parturition reproductive and urinary physiology Fetus lcsh:Veterinary medicine 030102 biochemistry & molecular biology General Veterinary medicine.disease Cell biology horse 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure lcsh:SF600-1100 Animal Science and Zoology |
Zdroj: | Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 675, p 675 (2021) Animals Volume 11 Issue 3 |
ISSN: | 2076-2615 |
Popis: | Retained fetal membranes (RFM) is one of the most common post-partum diseases of a complex etiology. Moreover, its pathogenesis is still not elucidated. Detailed transcriptomic analysis of physiological and retained placenta may bring profound insight in the pathogenesis of the disease. The aim of the study was to compare the transcriptome of the retained and physiologically released placenta as well as biological pathways and processes in order to determine the possible pathogenesis of the disease. Samples of the endometrium and the allantochorion were taken within 2 h after parturition from control mares (n = 3) and mares with RFM (n = 3). RNA sequencing was performed with the use of all samples and mRNA expression of chosen genes was validated with Real Time PCR. Analysis of RNA-seq identified 487 differentially expressed genes in the allantochorion and 261 in the endometrium of control and RFM mares (p < 0.0001). Within genes that may be important in the release of fetal membranes and were differentially expressed, our report pinpointed BGN, TIMP1, DRB, CD3E, C3, FCN3, CASP3, BCL2L1. Gene ontology analysis showed possible processes which were altered in RFM that are apoptosis, inflammatory-related processes, and extracellular matrix metabolism and might be involved in the pathogenesis of RFM. This is the first report on the transcriptome of RFM and physiologically released placenta in mares. |
Databáze: | OpenAIRE |
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