Biofilm inhibition and anti-quorum sensing activity of phytosynthesized silver nanoparticles against the nosocomial pathogen Pseudomonas aeruginosa
| Autor: | Shatavari Kulshrestha, Neelu Nawani, Swapnil Gaikwad, Viniti Vaidya, Sarika Pawar, Shuchi Nagar, Saloni Shah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Silver Virulence Factors Phytochemicals 030106 microbiology Metal Nanoparticles Virulence Microbial Sensitivity Tests Aquatic Science medicine.disease_cause Applied Microbiology and Biotechnology Silver nanoparticle Microbiology 03 medical and health sciences chemistry.chemical_compound medicine Water Science and Technology Cross Infection Pancreatic Elastase biology Plant Extracts Pseudomonas aeruginosa Chromobacterium Biofilm Quorum Sensing biology.organism_classification Anti-Bacterial Agents Piper betle Molecular Docking Simulation Plant Leaves Eugenol Quorum sensing 030104 developmental biology chemistry Phytochemical Biofilms Gentian Violet Chromobacterium violaceum |
| Zdroj: | Biofouling. 35:34-49 |
| ISSN: | 1029-2454 0892-7014 |
| DOI: | 10.1080/08927014.2018.1563686 |
| Popis: | Quorum sensing (QS), the communication signaling network, regulates biofilm formation and several virulence factors in Pseudomonas aeruginosa PAO1, a nosocomial opportunistic pathogen. QS is considered to be a challenging target for compounds antagonistic to virulent factors. Biologically synthesized silver nanoparticles (AgNPs) are reported as anti-QS and anti-biofilm drugs against bacterial infections. The present study reports on the synthesis and characterization of Piper betle (Pb) mediated AgNPs (Pb-AgNPs). The anti-QS activity of Pb-AgNPs against Chromobacterium violaceum and the potential effect of Pb-AgNPs on QS-regulated phenotypes in PAO1 were studied. FTIR analysis exhibited that Pb-AgNPs had been capped by phytochemical constituents of Pb. Eugenol is one of the active phenolic phytochemicals in Pb leaves, therefore molecular docking of eugenol-conjugated AgNPs on QS regulator proteins (LasR, LasI and MvfR) was performed. Eugenol-conjugated AgNPs showed considerable binding interactions with QS-associated proteins. These results provide novel insights into the development of phytochemically conjugated nanoparticles as promising anti-infective candidates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|