Reversible structural alterations of undifferentiated and differentiated human neuroblastoma cells induced by phorbol ester
| Autor: | C P Reboulleau, Jury M. Vasiliev, I S Tint, Edward M. Bonder, Gel'fand Im, H. H. Feder |
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| Rok vydání: | 1992 |
| Předmět: |
Neurite
Cellular differentiation Video microscopy In Vitro Techniques Biology Microfilament Neuroblastoma chemistry.chemical_compound Neurites Tumor Cells Cultured Humans Growth cone Cytoskeleton Protein Kinase C Protein kinase C Neurons Multidisciplinary Cell Differentiation Actins Cell biology Biochemistry chemistry Cell culture Phorbol Tetradecanoylphorbol Acetate Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:8160-8164 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Morphological alterations in the structure of undifferentiated and morphologically differentiated human neuroblastoma cells induced by phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, were examined by video microscopy and immunomorphology. In undifferentiated cells, PMA induced the formation of motile actin-rich lamellas and of stable cylindrical processes rich in microtubules. Formation of stable processes resulted either from the collapse of lamellas or the movement of the cell body away from the base of a process. In differentiated cells, PMA induced the rapid extension of small lamellas and subsequent formation of short-lived elongated processes from the lateral edges of neurites. Additionally, growth cones exhibited enhanced modulation in shape after PMA treatment. These reversible reorganizations were similar to the actinoplast-tubuloplast transformations exhibited by PMA-treated fibroblasts. We suggest that actinoplast-tubuloplast reorganizations play essential roles in morphogenesis where stable cytoplasmic extensions are induced by external stimuli. In particular, PMA-induced reorganizations of neural cells in culture may be a model for morphological modulations that occur in nerve tissue. |
| Databáze: | OpenAIRE |
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